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Human/Mouse/Rat SHIP2 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5389

R&D Systems, part of Bio-Techne
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AF5389
AF5389-SP

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

Product Specifications

Immunogen

E. coli-derived recombinant human SHIP2
Ala1106-Lys1258
Accession # O15357

Specificity

Detects human, mouse and rat SHIP2 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat SHIP2 Antibody

Detection of Human/Mouse/Rat SHIP2 antibody by Western Blot.

Detection of Human/Mouse/Rat SHIP2 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, HUVEC human umbilical vein endothelial cells, PT18 mouse mast/basophil cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat SHIP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5389) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SHIP2 at approximately 140 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
SHIP2 antibody in PC-3 Human Cell Line by Immunocytochemistry (ICC).

SHIP2 in PC‑3 Human Cell Line.

SHIP2 was detected in immersion fixed PC-3 human prostate cancer cell line using Sheep Anti-Human/Mouse/Rat SHIP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5389) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. This application has not yet been tested in mouse or rat samples.
SHIP2 antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P).

SHIP2 in Human Breast Cancer Tissue.

SHIP2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Sheep Anti-Human/Mouse/Rat SHIP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5389) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human/Mouse/Rat SHIP2 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed PC‑3 human prostate cancer cell line

Immunohistochemistry

3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, HUVEC human umbilical vein endothelial cells, PT18 mouse mast/basophil cell line, and Rat-2 rat embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SHIP2

SHIP2 (SH2 domain-containing inositol 5-phosphatase 2; also inositol polyphosphate phosphatase-like 1) is a member of the phosphatidylinositol/PtdIns 5-phosphatase family of enzymes. It is widely expressed and negatively regulates PI3 kinase pathways by hydrolyzing the 5-phosphate of PtdIns-3,4,5-triphosphate. Human SHIP2 is 1258 amino acids (aa) in length. It contains one SH2 domain (aa 21‑117), a Pro-rich region with an SH3 binding site (aa 935-1105), and a SAM (or sterile alpha-motif) domain that binds ARAP3 (aa 1196‑1258). SHIP2 is phosphorylated in response to growth factor activation on Tyr986, 1162 and 1358, and on Thr958, among other sites. Over aa 1106‑1258, human SHIP2 shares 95% aa identity with mouse SHIP2.

Long Name

SH2-containing Inositol-5'-Phosphatase 2

Alternate Names

INPPL1

Entrez Gene IDs

3636 (Human); 16332 (Mouse); 65038 (Rat)

Gene Symbol

INPPL1

UniProt

Additional SHIP2 Products

Product Documents for Human/Mouse/Rat SHIP2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse/Rat SHIP2 Antibody

For research use only

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