Human/Mouse/Rat SMURF2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6916
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 690638
Product Specifications
Immunogen
E. coli-derived recombinant human SMURF2
Met490-Glu748
Accession # Q9HAU4
Met490-Glu748
Accession # Q9HAU4
Specificity
Detects human SMURF2 in direct ELISAs and Western blots.
In Western blots, no cross-reactivity with
recombinant human SMURF1 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human/Mouse/Rat SMURF2 Antibody
Detection of Human, Mouse, and Rat SMURF2 by Western Blot.
Western blot shows lysates of MDA-MB-468 human breast cancer cell line, Neuro-2A mouse neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human SMURF2 Monoclonal Antibody (Catalog # MAB6916) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SMURF2 at approximately 93 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for Human/Mouse/Rat SMURF2 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: MDA‑MB‑468 human breast cancer cell line, Neuro‑2A mouse neuroblastoma cell line, and Rat‑2 rat embryonic fibroblast cell line
Sample: MDA‑MB‑468 human breast cancer cell line, Neuro‑2A mouse neuroblastoma cell line, and Rat‑2 rat embryonic fibroblast cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SMURF2
Long Name
SMAD Ubiquitination Regulatory Factor 2
Alternate Names
DKFZp686F0270, E3 ubiquitin ligase SMURF2, E3 ubiquitin-protein ligase SMURF2, EC 6.3.2, EC 6.3.2.-, hSMURF2, MGC138150, SMAD specific E3 ubiquitin protein ligase 2, SMAD ubiquitination regulatory factor 2, SMAD-specific E3 ubiquitin-protein ligase 2
Gene Symbol
SMURF2
UniProt
Additional SMURF2 Products
Product Documents for Human/Mouse/Rat SMURF2 Antibody
Product Specific Notices for Human/Mouse/Rat SMURF2 Antibody
For research use only
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