Human/Mouse/Rat Sortilin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3154
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Knockout Validated, Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 334708
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Sortilin
Ser78-Asn755
Accession # Q99523
Ser78-Asn755
Accession # Q99523
Specificity
Detects human, mouse, and rat Sortilin in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human/Mouse/Rat Sortilin Antibody
Detection of Human Sortilin by Western Blot.
Western blot shows lysates of human brain (cerebellum) tissue and human brain (hippocampus) tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human/Mouse/Rat Sortilin Monoclonal Antibody (Catalog # MAB3154) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Sortilin at approximately 95-105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Sortilin by Simple WesternTM.
Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for Sortilin at approximately 87 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat Sortilin Monoclonal Antibody (Catalog # MAB3154) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Mouse and Rat Sortilin by Western Blot.
Western blot shows lysates of mouse brain tissue and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Sortilin Monoclonal Antibody (Catalog # MAB3154) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Sortilin at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat Sortilin Antibody
Application
Recommended Usage
Knockout Validated
Sortilin
is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in
Sortilin knockout HeLa cell line.
Simple Western
10 µg/mL
Sample: Human brain (cerebellum) tissue
Sample: Human brain (cerebellum) tissue
Western Blot
0.25-1 µg/mL
Sample: Human brain (cerebellum) tissue, human brain (hippocampus) tissue, mouse brain (total) tissue, and rat brain (total) tissue
Sample: Human brain (cerebellum) tissue, human brain (hippocampus) tissue, mouse brain (total) tissue, and rat brain (total) tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Sortilin
Alternate Names
Gp95, Ntr3, SORT1
Gene Symbol
SORT1
UniProt
Additional Sortilin Products
Product Documents for Human/Mouse/Rat Sortilin Antibody
Product Specific Notices for Human/Mouse/Rat Sortilin Antibody
For research use only
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