Human/Mouse/Rat Teneurin-4 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6320
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Primate - Chlorocebus aethiops (African Green Monkey)
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Teneurin-4
Lys61-Lys340
Accession # Q6N022
Lys61-Lys340
Accession # Q6N022
Specificity
Detects human, mouse, and rat Teneurin-4 in Western blots and human Teneurin-4 in direct ELISAs. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human (rh) Teneurin-1 is observed, and less than 1% cross-reactivity with rhTeneurin-2 and rhTeneurin-3 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat Teneurin-4 Antibody
Detection of Human and Mouse Teneurin-4 by Western Blot.
Western blot shows lysates of SH-SY5Y human neuroblastoma cell line and L-929 mouse fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Teneurin-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6320) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for Teneurin-4 at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of Rat Teneurin-4 by Western Blot.
Western blot shows lysates of Rat cortical stem cell. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Teneurin-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6320) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for Teneurin-4 at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.Applications for Human/Mouse/Rat Teneurin-4 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: SH‑SY5Y human neuroblastoma cell line, L‑929 mouse fibroblast cell line, and Rat cortical stem cell
Sample: SH‑SY5Y human neuroblastoma cell line, L‑929 mouse fibroblast cell line, and Rat cortical stem cell
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Teneurin-4
Teneurin-4 (also Ten-m4, Doc4 and tenascin-M4) is a 250-300 kDa member of the tenascin family, teneurin subfamily of transmembrane (TM) molecules. It is a covalently-linked homodimer that is widely expressed in the embryo, and in adult, participates in cell-to-cell adhesion, and may communicate ER stress levels. Human Teneurin-4 is a 2769 amino acid (aa) type II TM glycoprotein. It contains a 345 aa cytoplasmic region (aa 1-345), plus a 2403 aa extracellular domain (ECD) (aa 367‑2769). The ECD possesses eight sequential EGF-like domains (aa 561-831), five NHL repeats, each of which form a beta-propeller (aa 1216-1566), and 23 YD/TyrAsp-containing repeats that bind carbohydrates. C-terminal cleavage generates a short 5 kDa, 41 aa peptide (aa 2726-2766) termed TCAP-1 that shows bioactivity. Teneurin-4 is hypothesized to form heterodimers with other teneurins. Over aa 61-340, human Teneurin-4 shares 95% aa identity with mouse Teneurin-4.
Alternate Names
ODZ4, Ten-M4, Tenascin-M4, Teneurin4, TNM4
Entrez Gene IDs
26011 (Human)
Gene Symbol
TENM4
UniProt
Additional Teneurin-4 Products
Product Documents for Human/Mouse/Rat Teneurin-4 Antibody
Product Specific Notices for Human/Mouse/Rat Teneurin-4 Antibody
For research use only
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