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Human/Mouse/Rat TrkB Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1494

R&D Systems, part of Bio-Techne
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MAB1494
MAB1494-SP

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 225105

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TrkB
Cys32-His429
Accession # P15209

Specificity

Detects mouse TrkB in direct ELISAs. Detects human, mouse, and rat TrkB in Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human TrkA, recombinant rat TrkA, or recombinant mouse TrkC is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Human/Mouse/Rat TrkB Antibody

Detection of Human, Mouse, and Rat TrkB antibody by Western Blot.

Detection of Human, Mouse, and Rat TrkB by Western Blot.

Western blot shows lysates of human brain (motor cortex) tissue, mouse brain (cortex) tissue, and rat brain (hippocampus) tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse/Rat TrkB Monoclonal Antibody (Catalog # MAB1494) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for TrkB at approximately 95 kDa and 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse TrkB antibody by Western Blot.

Detection of Mouse TrkB by Western Blot.

Western blot shows lysate of mouse brain (cortex) tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse/Rat TrkB Monoclonal Antibody (Catalog # MAB1494) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for TrkB at approximately 90-100 and 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat TrkB Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse spinal cord

Western Blot

2 µg/mL
Sample: Human brain (motor cortex) tissue, Mouse brain (cortex) tissue, and Rat brain (hippocampus) tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TrkB

The neurotrophins, including NGF, BDNF, NT-3 and NT-4/5, constitute a group of structurally related, secreted proteins that play an important role in the development and function of the nervous system. The biological activities of the neurotrophins are mediated by binding to and activating two unrelated receptor types: the p75 neurotrophin receptor (p75NTR) and the Trk family of receptor tyrosine kinases (1, 2). p75NTR is a member of the tumor necrosis factor receptor superfamily (TNFRSF) and has been designated TNFRSF16. It binds all neurotrophins with low affinity to transduce cellular signaling pathways that synergize with or antagonize those activated by the Trk receptors. Three Trk family proteins, TrkA, TrkB, and TrkC, exhibiting different ligand specificities, have been identified. TrkA binds NGF and NT-3, TrkB binds BDNF, NT-3 and NT-4/5, and TrkC only binds NT-3 (1‑2). All Trk family proteins share a conserved, complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain (3). Natural splice variants of the different Trks, lacking the first cysteine-rich domain, the first and second or all three of the leucine-rich motifs, or the tyrosine kinase domain, have been described (4). At the protein sequence level, human and mouse TrkB show 94% amino acid sequence identity (5‑6). The proteins also exhibit cross-species activity. The primary location of TrkB expression is in the central and peripheral nervous systems. Low level TrkB expression has also been observed in a wide variety of tissues outside the nervous system (6).

References

  1. Huang, E.J. and L.F. Reichardt (2003) Annu. Rev. Biochem. 72:609.
  2. Dechant, G. (2001) Cell Tissue Res. 305:229.
  3. Schneider, R. and M. Schweiger (1991) Oncogene 6:1807.
  4. Ninkina, N. et al. (1997) J. Biol. Chem. 272:13019.
  5. Nakagawara A. et al. (1995) Genomics. 25:538.
  6. Klein, R. et al. (1989) EMBO J. 8:3701.

Long Name

Neurotrophic Tyrosine Kinase Receptor B

Alternate Names

NTRK2

Entrez Gene IDs

4915 (Human); 18212 (Mouse); 25054 (Rat)

Gene Symbol

NTRK2

UniProt

Additional TrkB Products

Product Documents for Human/Mouse/Rat TrkB Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse/Rat TrkB Antibody

For research use only

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