Human/Mouse/Rat TrkB Antibody

Detection of Human TrkB by Immunocytochemistry/Immunofluorescence

BDNF and TrkB protein expression in primary and metastatic colorectal cancer.Representative images of immuno-reactive BDNF and TrkB protein expression in primary CRC and peritoneal metastasis are shown (original magnification: 100×). Anti TrkB antibody (R&D Systems, Foster City, CA, USA) detected both TrkB.FL and TrkB.T1 proteins, which was confirmed by Western blotting analysis. (A): The immunoreactive BDNF protein is located in the cytoplasm of the tumor cells of the primary CRC. (B): The immunoreactive BDNF protein is located in the cytoplasm of the tumor cells in the corresponding peritoneal metastasis. (C): The immunoreactive TrkB protein is located in the nucleus of the tumor cells of the primary CRC. (D): The immunoreactive TrkB protein is located in the nucleus of the tumor cells in the corresponding peritoneal metastasis. These expression patterns for the BDNF and TrkB proteins were confirmed in CRC patients (n = 5) whose primary and peritoneal metastatic nodules were available for immunohistochemistry. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24801982), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human TrkB by Immunocytochemistry/Immunofluorescence

BDNF and TrkB protein expression in primary and metastatic colorectal cancer.Representative images of immuno-reactive BDNF and TrkB protein expression in primary CRC and peritoneal metastasis are shown (original magnification: 100×). Anti TrkB antibody (R&D Systems, Foster City, CA, USA) detected both TrkB.FL and TrkB.T1 proteins, which was confirmed by Western blotting analysis. (A): The immunoreactive BDNF protein is located in the cytoplasm of the tumor cells of the primary CRC. (B): The immunoreactive BDNF protein is located in the cytoplasm of the tumor cells in the corresponding peritoneal metastasis. (C): The immunoreactive TrkB protein is located in the nucleus of the tumor cells of the primary CRC. (D): The immunoreactive TrkB protein is located in the nucleus of the tumor cells in the corresponding peritoneal metastasis. These expression patterns for the BDNF and TrkB proteins were confirmed in CRC patients (n = 5) whose primary and peritoneal metastatic nodules were available for immunohistochemistry. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24801982), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human TrkB by Western Blot

Expression of BDNF and TrkB in CRC cells in vitro.(A): RT-PCR analysis of BDNF and TrkB mRNA levels in CRC cell lines CaCO2 (lane: 1), DLD1 (lane: 2), HT29 (lane: 3), LoVo (lane: 4), and SW480 (lane: 5). GAPDH mRNA levels was used as an internal control. TrkB mRNA levels contain both TrkB.FL and TrkB.T1 mRNAs. (B): Western blotting analysis of BDNF and TrkB protein levels in CRC cell lines CaCO2 (lane: 1), DLD1 (lane: 2), HT29 (lane: 3), LoVo (lane: 4), and SW480 (lane: 5). The full-length TrkB (TrkB.FL, 145 kDa) and the truncated TrkB (TrkB.T1, 95 kDa) were detected. Actin was used as an internal control. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24801982), licensed under a CC-BY license. Not internally tested by R&D Systems.