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Human/Mouse/Rat TRRAP Antibody

R&D Systems, part of Bio-Techne | Catalog # AF7365

R&D Systems, part of Bio-Techne
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AF7365
AF7365-SP

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

Product Specifications

Immunogen

E. coli-derived recombinant human TRRAP
Asn3610-Leu3859, predicted
Accession # Q9Y4A5

Specificity

Detects human, mouse and rat TRRAP in Western blots. Detects recombinant human TRRAP in direct ELISAs.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat TRRAP Antibody

Detection of Human, Mouse, and Rat TRRAP antibody by Western Blot.

Detection of Human, Mouse, and Rat TRRAP by Western Blot.

Western blot shows lysates of MOLT-4 human acute lymphoblastic leukemia cell line, HeLa human cervical epithelial carcinoma cell line, mouse placenta tissue, mouse uterus tissue, and rat ovary tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human TRRAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7365) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TRRAP at approximately 440 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat TRRAP Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: MOLT‑4 human acute lymphoblastic leukemia cell line, HeLa human cervical epithelial carcinoma cell line, mouse placenta tissue, mouse uterus tissue, and rat ovary tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRRAP

TRRAP (Transformation/transcription domain-associated protein; also PAF350/400 and STAF40) is a 400-450 kDa member of the TRA1 subfamily, PI3/PI4 kinase family, ATM superfamily of molecules. It is a ubiquitously expressed nuclear protein that functions as a promoter of gene transcription. In particular, the activity of the E2F transcription factor family is regulated by the degree of histone acetylation. Deacetylation inhibits E2F-mediated transcription, while acetylation promotes gene transcription. TRRAP promotes histone acetylation by serving as a positive modulator/cofactor for GCN5 acetyltransferase activity. TRRAP also acts independently of GCN5. It serves as a recruiter for Skp1, a ubiquitin ligase that ubiquitates chromatin-bound beta-catenin, leading to a downregulation of Wnt-signaling activity. Human TRRAP is 3859 amino acids (aa) in length. It contains one Pro-rich region (aa 485-526), an NLS (aa 2047-2062), two FAT domains (aa 2704-3275 and 3827-3859) and a nonenzymatic PI3/PI4 kinase domain (aa 3528-3826). There are multiple potential isoform variants. They show various combinations of a deletion involving aa 1493-1510, an Ala substitution for aa 3001-3012, a 119 aa substitution for aa 3784-3859, and an 18 aa insertion after Asp3287. Over aa 3610-3859, human TRRAP shares 99% aa identity with mouse TRRAP.

Long Name

Transformation/Transcription Domain-associated Protein

Alternate Names

PAF400, STAF40, TR-AP, Tra1

Entrez Gene IDs

8295 (Human)

Gene Symbol

TRRAP

UniProt

Additional TRRAP Products

Product Documents for Human/Mouse/Rat TRRAP Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse/Rat TRRAP Antibody

For research use only

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