Human/Mouse/Rat Yes Antibody

Name: Human/Mouse/Rat Yes Antibody
Brand: R&D Systems
SKU: MAB32051

R&D Systems, part of Bio-Techne | Catalog # MAB32051

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Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # 339827

View all Yes Antibodies »

Product Summary for Human/Mouse/Rat Yes Antibody

Immunogen

E. coli-derived recombinant human Yes
Met1-Gly88
Accession # P07947.3

Specificity

Detects endogenous human, mouse, and rat Yes in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human/Mouse/Rat Yes Antibody

Detection of Human and Rat Yes by Western Blot.

Western blot shows lysates of Rat-2 rat embryonic fibroblast cell line and MDA-MB-468 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Yes Monoclonal Antibody (Catalog # MAB32051) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Yes at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Yes in Human Breast Cancer Tissue.

Yes was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 25 µg/mL Human/Mouse/Rat Yes Monoclonal Antibody (Catalog # MAB32051) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human/Mouse/Rat Yes Antibody

Application

Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion-fixed paraffin-embedded sections of human brain (cerebellum) and human breast cancer tissue

Western Blot

0.5 µg/mL
Sample: Rat-2 rat embryonic fibroblast cell line and MDA-MB-468 human breast cancer cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Yes

Yes is the cellular homolog of the oncogenic protein encoded by the Yamaguchi 73 and Esh avian sarcoma viruses. After Src, Yes is the most widely expressed member of the Src family of nonreceptor tyrosine kinases. Src family kinases regulate an array of cellular processes, including growth factor signaling, cytoskeleton dynamics, and cell proliferation.

Long Name

V-Yes-1 Yamaguchi Sarcoma Viral Oncogene Homolog 1

Alternate Names

YES1

Entrez Gene IDs

7525 (Human); 22612 (Mouse); 24884 (Rat)

Gene Symbol