Human/Mouse RCOR1/CoREST Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6047
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human RCOR1/CoREST
Ala397-Ser486
Accession # Q9UKL0
Ala397-Ser486
Accession # Q9UKL0
Specificity
Detects human and mouse RCOR1/CoREST in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse RCOR1/CoREST Antibody
Detection of Human and Mouse RCOR1/CoREST by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line and NIH-3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse RCOR1/CoREST Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6047) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for RCOR1/CoREST at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse RCOR1/CoREST Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and NIH‑3T3 mouse embryonic fibroblast cell line
Sample: Jurkat human acute T cell leukemia cell line and NIH‑3T3 mouse embryonic fibroblast cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RCOR1/CoREST
RCOR1 (REST [RE-1 silencing transcription factor] CORepressor 1; also CoREST) is a 64‑66 kDa member of the CoREST family of proteins. It is a nuclear protein that is ubiquitously expressed. RCOR1 serves as a molecular bridge, linking REST with Sin3, HDAC, LSD1, histone H3 K9 methyltransferases, and MeCP2. This creates a complex that represses the expression of neuron‑specific genes in nonneuronal cells, principally by coordinating the demethylation of Lys4 in histone H3. Human RCOR1 is 482 amino acids (aa) in length. It contains one ELM2 domain (aa 102‑157) that interacts with HDAC, and two SANT sequences (aa 187‑238 and 378‑429) that bind DNA tandem repeats. There are three potential RCOR1 splice variants. One contains a four aa substitution for aa 296‑482, a second shows an alternative start site 3 aa upstream of the standard site, while a third shares the same upstream start site accompanied by a 15 aa substitution for aa 471‑482. Over aa 394‑482, human RCOR1 shares 93% aa identity with mouse RCOR1.
Long Name
REST Corepressor 1
Alternate Names
CoREST, RCOR1
Entrez Gene IDs
23186 (Human)
Gene Symbol
RCOR1
UniProt
Additional RCOR1/CoREST Products
Product Documents for Human/Mouse RCOR1/CoREST Antibody
Product Specific Notices for Human/Mouse RCOR1/CoREST Antibody
For research use only
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