Human peripheral blood mononuclear cells (PBMC) lymphocytes either (A) untreated or (B) treated with PMA were stained with Mouse Anti-Human/Mouse RUNX3/CBFA3 APC-conjugated Monoclonal Antibody (Catalog # IC3765A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human/Mouse RUNX3/CBFA3 APC-conjugated Antibody

Application

Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells
Sample: Human peripheral blood mononuclear cells (PBMC) lymphocytes treated with PMA were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: RUNX3/CBFA3

RUNX3, also known as CBFA3, AML-2 or PEBP2-alpha C, is a member of the Runt domain family of nuclear transcriptional regulators. All of the RUNX proteins form dimers with CBF-beta. The Runt domain, amino acids (aa) 54-186, is required for DNA binding, while a pro/ser/thr-rich region (aa 191-415) transcriptionally activates target genes. Isoform 2 has an alternate 19 aa in place of the N-terminal 5 aa of isoform 1. The 415 aa Human RUNX3 shares 91% aa identity with mouse or rat RUNX3. RUNX3 is necessary for growth control of gastric epithelium, neurogenesis of dorsal root ganglia, and T cell differentiation. RUNX3 expression is frequently mutated in tumors and appears to be silenced by methylation.

Long Name

Runt-related Transcription Factor 3

Alternate Names

AML2, CBFA3, PEBP2A3, PEBP2AC

Entrez Gene IDs

864 (Human); 12399 (Mouse); 156726 (Rat)

Gene Symbol

RUNX3

UniProt

Q13761

Additional RUNX3/CBFA3 Products

Product Documents for Human/Mouse RUNX3/CBFA3 APC-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human/Mouse RUNX3/CBFA3 APC-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human/Mouse RUNX3/CBFA3 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC3765A

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse RUNX3/CBFA3 APC-conjugated Antibody

Detection of RUNX3/CBFA3 in Human PBMC lymphocytes by Flow Cytometry.