Human/Mouse SAM68 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5509
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human and mouse SAM68
Met108-Pro280
Accession # Q07666
Met108-Pro280
Accession # Q07666
Specificity
Detects human and mouse SAM68 in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse SAM68 Antibody
Detection of Human and Mouse SAM68 by Western Blot.
Western blot shows nuclear extracts of Raji human Burkitt's lymphoma cell line, Daudi human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse SAM68 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5509) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SAM68 at approximately 60-68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse SAM68 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Raji human Burkitt's lymphoma cell line, Daudi human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line
Sample: Raji human Burkitt's lymphoma cell line, Daudi human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SAM68
SAM68 (Src associated in mitosis 68 kDa; also KH domain-containing, RNA-binding, signal transduction-associated protein 1) is a 62‑68 kDa member of the KHDRBS family of proteins. It is associated with multiple receptor systems (i.e.- TCR and leptin), as an adaptor protein by its SH2 and SH3 domains, and binds to UAAA motifs in mRNA. Human SAM68 is 443 amino acids (aa) in length. It contains one SGQ region (aa 96‑157) and one KH domain (aa 171‑197). Post‑translational modification of SAM68 alters its partnership with other molecules. For example, methylation on ArgGly motifs reduce RNA binding, acetylation on multiple Lys residues increases RNA binding, and phosphorylation on Tyr promotes SH3 domain interaction.
Long Name
Src-associated in Mitosis 68 kDa Protein
Alternate Names
KHDRBS1, p62, p68
Gene Symbol
KHDRBS1
UniProt
Additional SAM68 Products
Product Documents for Human/Mouse SAM68 Antibody
Product Specific Notices for Human/Mouse SAM68 Antibody
For research use only
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