Human/Mouse SOD1/Cu-Zn SOD Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3418

R&D Systems, part of Bio-Techne
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AF3418
AF3418-SP
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Rat, Xenograft

Applications

Validated:

Simple Western, Western Blot

Cited:

Immunoassay, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all SOD1/Cu-Zn SOD Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human SOD1/Cu‑Zn SOD
Met1-Gln154
Accession # P00441

Specificity

Detects human and mouse SOD1/Cu‑Zn SOD in Western blots. Detects rat SOD1, but Catalog # AF3787 is recommended.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse SOD1/Cu-Zn SOD Antibody

Detection of Human/Mouse SOD1/Cu-Zn SOD antibody by Western Blot.

Detection of Human/Mouse SOD1/Cu‑Zn SOD by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL Goat Anti-Human/Mouse SOD1/Cu-Zn SOD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3418) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human SOD1, SOD2, and SOD3 (1 ng/lane) were included. A specific band for SOD1/Cu-Zn SOD was detected at approximately 16-19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Detection of Human SOD1/Cu-Zn SOD antibody by Simple WesternTM.

Detection of Human SOD1/Cu-Zn SOD by Simple WesternTM.

Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SOD1/Cu-Zn SOD at approximately 25 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse SOD1/Cu-Zn SOD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3418) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Human SOD1/Cu-Zn SOD antibody by Simple WesternTM.

Detection of Human SOD1/Cu‑Zn SOD by Simple WesternTM.

Simple Western lane view shows lysates of A549 human lung carcinoma cell line and SK-BR-3 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for SOD1/Cu-Zn SOD at approximately 24 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse SOD1/Cu-Zn SOD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3418) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human/Mouse SOD1/Cu-Zn SOD Antibody

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, A549 human lung carcinoma cell line, and SK‑BR‑3 human breast cancer cell line

Western Blot

0.2 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and NIH-3T3 mouse embryonic fibroblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using AF3418 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOD1/Cu-Zn SOD

Superoxide Dismutases (SODs), originally identified as Indophenoloxidase (IPO), are enzymes that catalyze the converversion of naturally-occuring but harmful superoxide radicals into molecular oxygen and hydrogen peroxide. Superoxide Dismutases 1, SOD1, also known as Cu/Zn SOD, soluble SOD, and IPO-A, is a soluble, cytoplasmic 16 kDa homodimer. Each SOD1 monomer binds one Cu2+ and Zn2+ ion. Three isozymes of SOD have been identified and are functionally related but have very modest sequence homology. SOD1 shares 23% and 27% sequence identity with SOD2 and SOD3, respectively. Mutations in SOD1 have been suggested to be the cause of familial amyotrophic lateral sclerosis (ALS). The ALS-causing mutations of SOD1 are scattered throughout the protein and provide no clear functional or structural clues to the underlying disease mechanism. The oligomerization hypothesis suggests that mutant SOD1 proteins become misfolded and consequently oligomerize into high molecular weight aggregates that result in the death of motor neurons. The oxidative damage hypothesis suggests that loss of function mutations in SOD1 result in the intracellular accumulation of the superoxide radical, leading to free radical-mediated damage, the release of cytochrome c, and apoptosis.

Long Name

Superoxide Dismutase-1

Alternate Names

Cu-Zn SOD, CuZn SOD, Ipo1, IPOA, SOD, cytosolic, SOD, Soluble

Entrez Gene IDs

6647 (Human); 20655 (Mouse); 24786 (Rat)

Gene Symbol

SOD1

UniProt

P00441

Additional SOD1/Cu-Zn SOD Products

Product Documents for Human/Mouse SOD1/Cu-Zn SOD Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse SOD1/Cu-Zn SOD Antibody

For research use only

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