Human/Mouse SR140 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB9897
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 983615
Product Specifications
Immunogen
Synthetic peptide containing human SR140 Peptide
Accession # O15042
Accession # O15042
Specificity
Detects human SR140 in direct ELISAs. Detects human and mouse SR140 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human/Mouse SR140 Antibody
Detection of Human and Mouse SR140 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HEK293T human embryonic kidney cell line, Jurkat human acute T cell leukemia cell line, and M1 mouse myeloid leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse SR140 Monoclonal Antibody (Catalog # MAB9897) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SR140 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse SR140 Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, HEK293T human embryonic kidney cell line, Jurkat human acute T cell leukemia cell line, and M1 mouse myeloid leukemia cell line
Sample: HeLa human cervical epithelial carcinoma cell line, HEK293T human embryonic kidney cell line, Jurkat human acute T cell leukemia cell line, and M1 mouse myeloid leukemia cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SR140
Long Name
U2-Associated Protein SR140
Alternate Names
FSAPa, U2-Associated SR140 Protein, U2SURP
Gene Symbol
U2SURP
UniProt
Additional SR140 Products
Product Documents for Human/Mouse SR140 Antibody
Product Specific Notices for Human/Mouse SR140 Antibody
For research use only
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