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Key Product Details

Species Reactivity

Human, Mouse

Applications

Flow Cytometry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Rat IgM Clone # MC-631

Product Specifications

Immunogen

Four to eight cell stage mouse embryos

Specificity

Recognizes a carbohydrate epitope of SSEA-3 (4, 5).

Clonality

Monoclonal

Host

Rat

Isotype

IgM

Scientific Data Images for Human/Mouse SSEA-3 Alexa Fluor® 488-conjugated Antibody

Detection of SSEA-3 antibody in BG01V Human Embryonic Stem Cells antibody by Flow Cytometry.

Detection of SSEA‑3 in BG01V Human Embryonic Stem Cells by Flow Cytometry.

BG01V human embryonic stem cells was stained with Rat Anti-Human/Mouse SSEA‑3 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB1434G, filled histogram) or isotype control antibody (open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of SSEA-3 antibody in NTera-2 Human Cell Line antibody by Flow Cytometry.

Detection of SSEA‑3 in NTera‑2 Human Cell Line by Flow Cytometry.

NTera‑2 human testicular embryonic carcinoma cell line was stained with Rat Anti-Human/Mouse SSEA‑3 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB1434G, filled histogram) or isotype control antibody (open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human/Mouse SSEA-3 Alexa Fluor® 488-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

5 µL/106 cells
Sample: BG01V human embryonic stem cells and NTera‑2 human testicular embryonic carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgM-specific Affinity-purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: SSEA-3

SSEA-3, also known as glycolipid GB5, is expressed on the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG), and human embryonic stem cells (ES) (1). Expression of SSEA-3 is down regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-3 expression (2, 3).

References

  1. Zhou, D. et al. (2000) J. Biol. Chem. 275:22631.
  2. Thomson, J.A. and J.S. Odorico (2000) Trends Biotechnol. 18:53.
  3. Draper, J.S. et al. (2002) J. Anat. 200:249.
  4. Shevinsky, L.H. et al. (1982) Cell 30:697.
  5. Kannagi, R. et al. (1983) EMBO J. 2:2355.

Long Name

Stage-specific Embryonic Antigen-3

Alternate Names

SSEA3

Additional SSEA-3 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse SSEA-3 Alexa Fluor® 488-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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