Human/Mouse STAT4 Antibody
R&D Systems, part of Bio-Techne | Catalog # PA-ST4
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Chromatin Immunoprecipitation (ChIP)
Label
Unconjugated
Antibody Source
Polyclonal Rabbit Serum
Product Specifications
Specificity
Detects human and murine STAT4. Does not cross-react with any of the other STAT proteins.
Clonality
Polyclonal
Host
Rabbit
Isotype
Serum
Scientific Data Images for Human/Mouse STAT4 Antibody
Immunoprecipitation of Human STAT4.
STAT4 was immunoprecipitated from lysates (1 x 106- 107cells) of human peripheral blood lymphocytes (PBL) following incubation with 3 µg Human/Mouse STAT4 Polyclonal Antibody (Catalog # PA-ST4) for 1 hour at 2 - 8 ºC. STAT4-antibody complexes were absorbed using Protein A Immunoprecipitin (Life Technologies). Immunoprecipitated STAT4 was detected by Western blot using a 1:2000 dilution of Human/Mouse STAT4 Polyclonal Antibody (Catalog # PA-ST4). View our recommended buffer recipes for immunoprecipitation.Applications for Human/Mouse STAT4 Antibody
Application
Recommended Usage
Immunoprecipitation
3 µg/500 µg cell lysate
Sample: Human peripheral blood lymphocytes, see our available Western blot detection antibodies
Sample: Human peripheral blood lymphocytes, see our available Western blot detection antibodies
Western Blot
1:2000 dilution
Sample: STAT4 immunoprecipitated from lysates of human peripheral blood lymphocytes
Sample: STAT4 immunoprecipitated from lysates of human peripheral blood lymphocytes
Formulation, Preparation, and Storage
Formulation
Lyophilized from 0.2 mL antiserum containing 0.02% sodium azide.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: STAT4
Signal transduction and activator of transcription 4 (STAT4) is a member of the Jak/STAT signal transduction pathway. STAT4 mediates cytokine signaling by acting as a signal transducer in the cytoplasm and, upon phosphorylation, homo or heterodimerizes with other STAT proteins, which then translocate to the nucleus and activate transcription of specific genes. STAT4 is expressed in subset of tissues including spleen, heart, brain, peripheral blood cells and testis. STAT4 is involved in mediating IL-12 response in lymphocytes and regulating T helper cell differentiation.
Long Name
Signal Transducer and Activator of Transcription 4
Alternate Names
signal transducer and activator of transcription 4, SLEB11
Gene Symbol
STAT4
Additional STAT4 Products
Product Documents for Human/Mouse STAT4 Antibody
Product Specific Notices for Human/Mouse STAT4 Antibody
For research use only
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