Human/Mouse STAT6 aa 342-640 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF21671
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse
Applications
Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Product Specifications
Immunogen
E. coli-derived recombinant human STAT6
Asn342-Gly640
Accession # P42226
Asn342-Gly640
Accession # P42226
Specificity
Detects human and mouse STAT6 in Western blots.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human/Mouse STAT6 aa 342-640 Antibody
Detection of Human STAT6 by Western Blot.
Western blot shows lysates of U937 human histiocytic lymphoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IL-4 (Catalog # 204-IL) for 30 minutes. Gels were loaded with 20 µg of cytoplasmic (Cyto) and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse STAT6 aa 342-640 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF21671) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band for STAT6 was detected at approximately 110 kDa (as indicated). For additional reference, duplicate lysates were probed with 1.0 µg/mL Human Phospho-STAT6 (Y641) Antigen Affinity-purified Polyclonal Antibody (lower panel, AF3717). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human STAT6 by Simple WesternTM.
Simple Western shows lysates of Daudi human Burkitt's lymphoma cell line, loaded at 0.5 mg/ml. A specific band was detected for STAT6 at approximately 99 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human/Mouse STAT6 aa 342-640 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF21671). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Mouse STAT6 aa 342-640 Antibody
Application
Recommended Usage
Simple Western
1 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line
Sample: Daudi human Burkitt's lymphoma cell line
Western Blot
0.2 µg/mL
Sample: U937 human histiocytic lymphoma cell line treated with Recombinant Human IL‑4 (Catalog # 204-IL)
Sample: U937 human histiocytic lymphoma cell line treated with Recombinant Human IL‑4 (Catalog # 204-IL)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS and Sodium Azide with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: STAT6
Signal Transducer and Activator of Transcription 6 (STAT6) is a member of the Jak/STAT signal transduction pathway and mediates cytokine signaling by IL‑4. IL‑4 activation leads to tyrosine phosphorylation and accumulation in the nucleus of STAT6 homo and heterodimers. STAT6 binds to the consensus DNA binding site TTCN4GAA. STAT6 transcriptional activation occurs by binding p100 and p160 class (NcoA1, Src-1) co-activators. Gene expression studies indicate that STAT6 mediates cell proliferation and anti-apoptotic effects of IL‑4. Also, knockout studies in mice suggest that STAT6 functions in T helper 2 (Th2) cell differentiation and transcriptional expression of cell surface markers and class switch of immunoglobulins.
Long Name
Signal Transducer and Activator of Transcription 6
Alternate Names
D12S1644, EC 2.4.1.227, EC 2.7.7.6, IL-4 Stat, IL-4-STAT, signal transducer and activator of transcription 6, signal transducer and activator of transcription 6, interleukin-4 induced, STAT, interleukin-4 induced, STAT, interleukin4-induced, STAT6B, STAT6C, transcription factor IL-4 STAT
Gene Symbol
STAT6
UniProt
Additional STAT6 Products
Product Documents for Human/Mouse STAT6 aa 342-640 Antibody
Product Specific Notices for Human/Mouse STAT6 aa 342-640 Antibody
For research use only
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