Human/Mouse STAT6 Antibody

R&D Systems, part of Bio-Techne | Catalog # PA-ST6

R&D Systems, part of Bio-Techne
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PA-ST6
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit Serum

View all STAT6 Antibodies »

Product Specifications

Specificity

Detects human and murine STAT 6. This antiserum does not crossreact with any of the other STAT proteins.

Clonality

Polyclonal

Host

Rabbit

Isotype

Serum

Scientific Data Images for Human/Mouse STAT6 Antibody

Detection of Human STAT6 antibody by Western Blot.

Detection of Human STAT6 by Western Blot.

Western blot shows lysates of TF-1 human erythroleukemic cell line and human peripheral blood lymphocyte. PVDF membrane was probed with 1:2000 dilution of Human/Mouse STAT6 Polyclonal Antibody (Catalog # PA-ST6) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for STAT6 at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 9.

Applications for Human/Mouse STAT6 Antibody

Application
Recommended Usage

Immunoprecipitation

3-6 µg/106 cells
Sample: TF-1 human erythroleukemic cell line, see our available Western blot detection antibodies

Western Blot

2000x µg/mL
Sample: TF-1 human erythroleukemic cell line and human peripheral blood lymphocyte
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Lyophilized from 0.2 mL antiserum containing 0.02% sodium azide

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: STAT6

Signal Transducer and Activator of Transcription 6 (STAT6) is a member of the Jak/STAT signal transduction pathway and mediates cytokine signaling by IL-4. IL-4 activation leads to tyrosine phosphorylation and accumulation in the nucleus of STAT6 homo and heterodimers. STAT6 acts as a signal transducer in the cytoplasm and, upon phosphorylation at Y641, translocates to the nucleus and binds to the DNA consensus site TTCN4GAA. Knockout studies in mice suggest that STAT6 functions in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins. STAT6 transcriptional activation occurs by binding p100 and p160 class (NcoA1, Src-1) co-activators. Gene expression studies indicate that STAT6 mediates cell proliferation and anti-apoptotic effects of IL-4.

Long Name

Signal Transducer and Activator of Transcription 6

Alternate Names

D12S1644, EC 2.4.1.227, EC 2.7.7.6, IL-4 Stat, IL-4-STAT, signal transducer and activator of transcription 6, signal transducer and activator of transcription 6, interleukin-4 induced, STAT, interleukin-4 induced, STAT, interleukin4-induced, STAT6B, STAT6C, transcription factor IL-4 STAT

Entrez Gene IDs

6778 (Human); 20852 (Mouse); 362896 (Rat)

Gene Symbol

STAT6

Additional STAT6 Products

Product Documents for Human/Mouse STAT6 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse STAT6 Antibody

For research use only

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