Human/Mouse TGF-beta  RI/ALK-5 Antibody

Detection of Mouse TGF-beta RI/ALK-5 by Western Blot

TGFRI and TGFRII association in fibulin-6 KD cells upon TGF-beta stimulus.scr siRNA or fibulin-6 siRNA transfected nCF, after TGF-beta stimulation are subjected to (a) FACS analysis. No difference in the amount of TGF betaRI and TGF betaRII on the surface of control and fibulin-6 KD cells was observed. (b) Immuno-precipitation of TGFRII from cell lysates of scr transfected and TGF-beta stimulated cells, shows more accumulation of TGFRI after western blotting compared to non TGF-beta treated nCF. (c) Immuno-precipitation of TGFRII followed by WB for TGFRI from cell lysates of scr transfected or fibulin-6 KD cells after TGF-beta stimulation display reduced association of receptors in fibulin-6 KD condition. The control input lanes shows no difference and IgG control is also clean (n = 4, p < 0.05). (d) Phosphorylation status of TGFRI was analyzed by western blot using phospho-TGFRI specific antibody. Densitometric analysis of western blot display decreased phosphorylation of TGFRI in fibulin-6 KD cells after TGF-beta stimulation compared to control cells (n = 5, p < 0.01, nonparametric Mann-Whitney U test). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28209981), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse TGF-beta RI/ALK-5 by Western Blot

TGFRI and TGFRII association in fibulin-6 KD cells upon TGF-beta stimulus.scr siRNA or fibulin-6 siRNA transfected nCF, after TGF-beta stimulation are subjected to (a) FACS analysis. No difference in the amount of TGF betaRI and TGF betaRII on the surface of control and fibulin-6 KD cells was observed. (b) Immuno-precipitation of TGFRII from cell lysates of scr transfected and TGF-beta stimulated cells, shows more accumulation of TGFRI after western blotting compared to non TGF-beta treated nCF. (c) Immuno-precipitation of TGFRII followed by WB for TGFRI from cell lysates of scr transfected or fibulin-6 KD cells after TGF-beta stimulation display reduced association of receptors in fibulin-6 KD condition. The control input lanes shows no difference and IgG control is also clean (n = 4, p < 0.05). (d) Phosphorylation status of TGFRI was analyzed by western blot using phospho-TGFRI specific antibody. Densitometric analysis of western blot display decreased phosphorylation of TGFRI in fibulin-6 KD cells after TGF-beta stimulation compared to control cells (n = 5, p < 0.01, nonparametric Mann-Whitney U test). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28209981), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse TGF-beta RI/ALK-5 by Flow Cytometry

TGFRI and TGFRII association in fibulin-6 KD cells upon TGF-beta stimulus.scr siRNA or fibulin-6 siRNA transfected nCF, after TGF-beta stimulation are subjected to (a) FACS analysis. No difference in the amount of TGF betaRI and TGF betaRII on the surface of control and fibulin-6 KD cells was observed. (b) Immuno-precipitation of TGFRII from cell lysates of scr transfected and TGF-beta stimulated cells, shows more accumulation of TGFRI after western blotting compared to non TGF-beta treated nCF. (c) Immuno-precipitation of TGFRII followed by WB for TGFRI from cell lysates of scr transfected or fibulin-6 KD cells after TGF-beta stimulation display reduced association of receptors in fibulin-6 KD condition. The control input lanes shows no difference and IgG control is also clean (n = 4, p < 0.05). (d) Phosphorylation status of TGFRI was analyzed by western blot using phospho-TGFRI specific antibody. Densitometric analysis of western blot display decreased phosphorylation of TGFRI in fibulin-6 KD cells after TGF-beta stimulation compared to control cells (n = 5, p < 0.01, nonparametric Mann-Whitney U test). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28209981), licensed under a CC-BY license. Not internally tested by R&D Systems.