Human/Mouse TGF-beta  RI/ALK-5 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5871R

Recombinant Monoclonal Antibody.
R&D Systems, part of Bio-Techne
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MAB5871R-100
MAB5871R-SP
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rat IgG2A Clone # 141231R

View all TGF-beta RI/ALK-5 Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse TGF‑ beta RI/ALK-5
Ala21-Glu121
Accession # BAA05023

Specificity

Detects mouse TGF‑ beta RI/ALK-5 in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Human/Mouse TGF-beta  RI/ALK-5 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human TGF-beta  RI/ALK‑5 Fc Chimera (Catalog # 3025-BR) and Recombinant Mouse TGF-beta  RI/ALK‑5 Fc Chimera (Catalog # 587-RI)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGF-beta RI/ALK-5

Most cell types express three sizes of receptors for TGF-beta. These are designated Type I (53 kDa), Type II (70-85 kDa), and Type III (250-350 kDa). The Type III receptor, a proteoglycan that exists in membrane-bound and soluble forms, binds TGF-beta 1, TGF-beta 2, and TGF-beta 3 but does not appear to be involved in signal transduction. The Type II receptor is a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The Type I receptor, originally known as ALK-5 (Activin receptor‑like kinase) is also a membrane-bound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGF-beta. Current evidence suggests that signal transduction requires the cytoplasmic domains of both the Type I and Type II receptors.

References

  1. Miyazono, K. et al. (1994) Adv. In Immunol. 55:181.
  2. Massagùe, J. (1998) Ann. Rev. Biochem. 67:753.

Long Name

Transforming Growth Factor beta Receptor I

Alternate Names

ALK-5, SKR4, TGF-bRI, TGFbetaRI, TGFBR1

Entrez Gene IDs

7046 (Human); 21812 (Mouse); 29591 (Rat)

Gene Symbol

TGFBR1

UniProt

BAA05023

Additional TGF-beta RI/ALK-5 Products

Product Documents for Human/Mouse TGF-beta  RI/ALK-5 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse TGF-beta  RI/ALK-5 Antibody

For research use only

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