Human/Mouse TOR Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1537
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry
Cited:
Immunohistochemistry, Infared Microscopy, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 303728
Product Specifications
Immunogen
E. coli-derived recombinant human TOR
Phe1720-Ala2020
Accession # P42345
Phe1720-Ala2020
Accession # P42345
Specificity
Detects human TOR in direct ELISAs.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human/Mouse TOR Antibody
TOR in MCF-7 Human Cell Line.
TOR was detected in immersion fixed MCF-7 human breast cancer cell line using 10 µg/mL Human/Mouse TOR Monoclonal Antibody (Catalog # MAB1537) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human/Mouse TOR Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed MCF-7 human breast cancer cell line
Sample: Immersion fixed MCF-7 human breast cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB1537 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TOR
Long Name
Mammalian Target of Rapamycin
Alternate Names
FRAP1, FRAP2, MTOR, RAFT1
Gene Symbol
MTOR
UniProt
Additional TOR Products
Product Documents for Human/Mouse TOR Antibody
Product Specific Notices for Human/Mouse TOR Antibody
For research use only
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