Human/Mouse TORC2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6338
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 628430
Product Specifications
Immunogen
E. coli-derived recombinant human TORC2
Lys453-Ser613
Accession # Q53ET0
Lys453-Ser613
Accession # Q53ET0
Specificity
Detects human and mouse TORC2 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse TORC2 Antibody
Detection of Human and Mouse TORC2 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, U2OS human osteosarcoma cell line, MDA-MB-453 human breast cancer cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 0.01 µg/mL of Mouse Anti-Human/Mouse TORC2 Monoclonal Antibody (Catalog # MAB6338) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TORC2 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse TORC2 Antibody
Application
Recommended Usage
Western Blot
0.01 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, U2OS human osteosarcoma cell line, MDA‑MB‑453 human breast cancer cell line, and NIH‑3T3 mouse embryonic fibroblast cell line
Sample: HeLa human cervical epithelial carcinoma cell line, U2OS human osteosarcoma cell line, MDA‑MB‑453 human breast cancer cell line, and NIH‑3T3 mouse embryonic fibroblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TORC2
TORC2, also known as CREB-regulated transcription co-activator 1 (crtc2), is an approximately 75 kDa protein that functions as a co‑activator for CREB1 in promoting transcription through both consensus and variant cAMP response element (CRE) sites. TORC2 activity is important in regulating the expression of genes involved in cellular energy metabolism. Its O-glycosylation triggers TORC2-dependent induction of hepatic gluconeogenic enzymes. It mediates hypothalamic glucose sensing and also the sensing of endoplasmic reticulum stress. In addition, TORC2 contributes to the transcription of HTLV-1 genes and the activation of latent EBV infections. Within aa 453‑613, human TORC2 shares 84% aa sequence identity with mouse TORC2.
Long Name
Transducer of Regulated CREB protein 1
Alternate Names
CRTC2
Gene Symbol
CRTC2
UniProt
Additional TORC2 Products
Product Documents for Human/Mouse TORC2 Antibody
Product Specific Notices for Human/Mouse TORC2 Antibody
For research use only
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