Human/Mouse VAMP-1/VAMP-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5958
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 540524
Product Summary for Human/Mouse VAMP-1/VAMP-2 Antibody
Immunogen
E. coli-derived recombinant human VAMP-1
Met1-Lys96
Accession # P23763
Met1-Lys96
Accession # P23763
Specificity
Detects human and mouse VAMP-1/VAMP-2 in Western blots. This antibody does not react with recombinant VAMP family members 5, 7, and 8.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human/Mouse VAMP-1/VAMP-2 Antibody
Detection of Human/Mouse VAMP-1/VAMP-2 by Western Blot.
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, HepG2 human hepatocellular carcinoma cell line, L1.2 mouse pro-B cell line, and BaF3 mouse pro-B cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse VAMP-1/VAMP-2 Monoclonal Antibody (Catalog # MAB5958) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for VAMP-1/VAMP-2 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse VAMP-1/VAMP-2 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (globus pallidus)
Sample: Immersion fixed paraffin-embedded sections of human brain (globus pallidus)
Western Blot
0.2 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line, HepG2 human hepatocellular carcinoma cell line, L1.2 mouse pro-B cell line, and BaF3 mouse pro-B cell line
Sample: Daudi human Burkitt's lymphoma cell line, HepG2 human hepatocellular carcinoma cell line, L1.2 mouse pro-B cell line, and BaF3 mouse pro-B cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB5958 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VAMP-1/VAMP-2
Long Name
Vesicle-Associated Membrane Protein 1/2
Alternate Names
SPAX1, SYB1, VAMP1, VAMP-1, vesicle associated membrane protein 1
Gene Symbol
VAMP1
UniProt
Additional VAMP-1/VAMP-2 Products
Product Documents for Human/Mouse VAMP-1/VAMP-2 Antibody
Product Specific Notices for Human/Mouse VAMP-1/VAMP-2 Antibody
For research use only
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