Human/Mouse Vav-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3786
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 591529
Product Specifications
Immunogen
E. coli-derived recombinant human Vav‑1
Trp25-Leu228
Accession # P15498
Trp25-Leu228
Accession # P15498
Specificity
Detects human and mouse Vav‑1 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse Vav-1 Antibody
Detection of Human and Mouse Vav‑1 by Western Blot.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, and CH-1 mouse B cell lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse Vav-1 Monoclonal Antibody (Catalog # MAB3786) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Vav-1 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 10.Applications for Human/Mouse Vav-1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Raji human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, and CH‑1 mouse B cell lymphoma cell line
Sample: Raji human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, and CH‑1 mouse B cell lymphoma cell line
Reviewed Applications
Read 1 review rated 2 using MAB3786 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Vav-1
The Vav-1 proto-oncogene is a guanine nucleotide exchange factor (GEF) for the low molecular weight GTPases of the Rho/Rac family. Vav-1 promotes intracellular signaling by its multiple protein binding motifs. It is a cytosolic protein primarily expressed in hematopoietic cells and plays an essential role in the proliferation and activation of T and B cells.
Alternate Names
vav-T, Vav1
Gene Symbol
VAV1
UniProt
Additional Vav-1 Products
Product Documents for Human/Mouse Vav-1 Antibody
Product Specific Notices for Human/Mouse Vav-1 Antibody
For research use only
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