Human/Mouse WASP Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3070
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human WASP
Lys230-Gln310
Accession # P42768
Lys230-Gln310
Accession # P42768
Specificity
Detects human and mouse WASP in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse WASP Antibody
Detection of Human, Mouse and Rat WASP by Western Blot.
Western blot shows lysates of NRK rat normal kidney cell, K562 human chronic myelogenous leukemia cell line, and Balb/3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse WASP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3070) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for WASP at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
WASP in Jurkat Human Cell Line.
WASP was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Goat Anti-Human/Mouse WASP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3070) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human/Mouse WASP Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells and immersion fixed Jurkat human acute T cell leukemia cell line
Sample: Immersion fixed human peripheral blood mononuclear cells and immersion fixed Jurkat human acute T cell leukemia cell line
Western Blot
0.5 µg/mL
Sample: NRK rat normal kidney cell line, K562 human chronic myelogenous leukemia cell line, and Balb/3T3 mouse embryonic fibroblast cell line
Sample: NRK rat normal kidney cell line, K562 human chronic myelogenous leukemia cell line, and Balb/3T3 mouse embryonic fibroblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: WASP
Long Name
Wiskott-Aldrich Syndrome Protein
Alternate Names
Thrombocytopenia 1, WAS
Gene Symbol
WAS
UniProt
Additional WASP Products
Product Documents for Human/Mouse WASP Antibody
Product Specific Notices for Human/Mouse WASP Antibody
For research use only
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