Human/Mouse Wnt-2b Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3900
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse Wnt-2b
Leu64-Glu104 and Arg251-Ala312
Accession # O70283
Leu64-Glu104 and Arg251-Ala312
Accession # O70283
Specificity
Detects recombinant human and recombinant mouse Wnt-2b in direct ELISAs and Western blots. In Western blots, approximately 20% cross-reactivity with recombinant human (rh) Wnt-2 is observed and less than 5% cross-reactivity with rhWnt-7a, -9a, recombinant mouse Wnt-1, -3a, -4, -5a, -5b, -8a, -8b, -9b, -10a, -10b, -11, and -16 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human/Mouse Wnt-2b Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed BT-20 human breast cancer cell line
Sample: Immersion fixed BT-20 human breast cancer cell line
Western Blot
0.1 µg/mL
Sample: Recombinant Human Wnt‑2b
Recombinant Mouse Wnt‑2b
Sample: Recombinant Human Wnt‑2b
Recombinant Mouse Wnt‑2b
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Wnt-2b
Mouse Wnt-2b (also Wnt-13) is a 389 amino acid, 42kDa member of the large and highly conserved Wnt family of secreted, cysteine-rich glycoprotein signaling molecules. Wnt proteins are associated with developmental and carcinogenic processes. Wnt-2b may be necessary to maintain stem cells in an undifferentiated state. As a type II transmembrane protein with a long C-terminal extracellular and a short N-terminal cytoplasmic domain, Wnt-2b is presumably cleaved within its extracellular region to release the secreted form. In the region from aa 64‑104, mouse Wnt-2b is 100% identical to the human and rat protein, and over the 251‑312 aa region also used as immunogen, mouse Wnt-2b is 97% and 100% identical to the corresponding human and rat sequences, respectively.
Long Name
Wingless-type MMTV Integration Site Family Member 2b
Alternate Names
Wnt-13, Wnt2b, XWNT2
Gene Symbol
WNT2B
UniProt
Additional Wnt-2b Products
Product Documents for Human/Mouse Wnt-2b Antibody
Product Specific Notices for Human/Mouse Wnt-2b Antibody
For research use only
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