Mouse Wnt-2b Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF3900
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse Wnt-2b
Leu64-Glu104 and Arg251-Ala312
Accession # O70283
Leu64-Glu104 and Arg251-Ala312
Accession # O70283
Specificity
Detects mouse Wnt-2b in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant human (rh) Wnt-2 is observed and less than 5% cross-reactivity with rhWnt-7a, rhWnt-9a, recombinant mouse (rm) Wnt-1, rmWnt-3a, rmWnt-4, rmWnt-5a, rmWnt-5b, rmWnt-8a, rmWnt-8b, rmWnt-9b, rmWnt-10a, rmWnt-10b, rmWnt-11, and rmWnt-16 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse Wnt-2b Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Wnt-2b
Sample: Recombinant Mouse Wnt-2b
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Wnt-2b
Mouse Wnt-2b (also Wnt-13) is a 389 amino acid, 42kDa member of the large and highly conserved Wnt family of secreted, cysteine-rich glycoprotein signaling molecules. Wnt proteins are associated with developmental and carcinogenic processes. Wnt-2b may be necessary to maintain stem cells in an undifferentiated state. As a type II transmembrane protein with a long C-terminal extracellular and a short N-terminal cytoplasmic domain, Wnt-2b is presumably cleaved within its extracellular region to release the secreted form. In the region from aa 64‑104, mouse Wnt-2b is 100% identical to the human and rat protein, and over the 251‑312 aa region also used as immunogen, mouse Wnt-2b is 97% and 100% identical to the corresponding human and rat sequences, respectively.
Long Name
Wingless-type MMTV Integration Site Family Member 2b
Alternate Names
Wnt-13, Wnt2b, XWNT2
Gene Symbol
WNT2B
UniProt
Additional Wnt-2b Products
Product Documents for Mouse Wnt-2b Biotinylated Antibody
Product Specific Notices for Mouse Wnt-2b Biotinylated Antibody
For research use only
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