Human/Mouse XIAP Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF8221
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human XIAP
Met1-Ser497 (Ser162Cys)
Accession # P98170
Met1-Ser497 (Ser162Cys)
Accession # P98170
Specificity
Detects human and mouse XIAP in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse XIAP Biotinylated Antibody
Detection of Human and Mouse XIAP by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human XIAP Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF8221) followed by Streptavidin-HRP (Catalog # DY998). A specific band was detected for XIAP at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for Human/Mouse XIAP Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.25 µg/mL
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: XIAP
XIAP (X-chromosome linked inhibitor of apoptosis) is a member of the apoptosis (IAP) family of proteins that inhibit caspases. The BIR2 domain of XIAP inhibits caspase-3 and caspase-7. The ability of XIAP to inhibit caspases is prevented by SMAC/Diablo through binding to XIAP-BIR2 and -BIR3 domains.
Long Name
X-linked Inhibitor of Apoptosis
Alternate Names
BIRC4
Gene Symbol
XIAP
UniProt
Additional XIAP Products
Product Documents for Human/Mouse XIAP Biotinylated Antibody
Product Specific Notices for Human/Mouse XIAP Biotinylated Antibody
For research use only
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