Human/Mouse ZBTB7A/Pokemon Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3496
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 466407
Product Specifications
Immunogen
E. coli-derived recombinant human ZBTB7A/Pokemon
Met349-Val476
Accession # O95365
Met349-Val476
Accession # O95365
Specificity
Detects human and mouse ZBTB7A/Pokemon in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse ZBTB7A/Pokemon Antibody
Detection of Human and Mouse ZBTB7A/Pokemon by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse ZBTB7A/Pokemon Monoclonal Antibody (Catalog # MAB3496) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ZBTB7A/Pokemon at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 10.Applications for Human/Mouse ZBTB7A/Pokemon Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, and NIH‑3T3 mouse embryonic fibroblast cell line
Sample: HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, and NIH‑3T3 mouse embryonic fibroblast cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ZBTB7A/Pokemon
ZBTB7A (zinc finger and BTB domain-containing 7A), also known as Pokemon, FBI-1 in human, LRF in mouse, and OCZF in rat, is a widely expressed nuclear protein that is overexpressed in many human cancers. It is a transcriptional repressor of ARF, c-fos, c-myc, Rb and others, including many extracellular matrix genes. These targets play important roles, both in normal differentiation and oncogenesis. Human ZBTB7A/Pokemon is a 584 amino acid (aa) protein with a BTB-POZ domain (aa 24‑131) and four zinc finger repeats (aa 382‑484). Human ZBTB7A/Pokemon shares 98% aa identity with mouse and rat within aa 349‑476.
Long Name
Zinc Finger and BTB Domain-Containing Protein 7A
Alternate Names
FBI-1, LRF, Pokemon, TIP21
Gene Symbol
ZBTB7A
UniProt
Additional ZBTB7A/Pokemon Products
Product Documents for Human/Mouse ZBTB7A/Pokemon Antibody
Product Specific Notices for Human/Mouse ZBTB7A/Pokemon Antibody
For research use only
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