Human MS4A1/CD20 APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB4225A
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 396444
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human MS4A1/CD20
Met1-Pro297
Accession # P11836
Met1-Pro297
Accession # P11836
Specificity
Detects human MS4A1/CD20. Stains human MS4A1/CD20 transfectants but not irrelevant transfectants.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human MS4A1/CD20 APC-conjugated Antibody
Detection of MS4A1/CD20 in Human Peripheral Blood Lymphocytes by Flow Cytometry.
Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 PE-conjugated Monoclonal Antibody (Catalog # FAB4867P) and either (A) Mouse Anti-Human MS4A1/CD20 APC-conjugated Monoclonal Antibody (Catalog # FAB4225A) or (B) Mouse IgG1Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.
Detection of MS4A1/CD20 in HEK293 Human Cell Line Transfected with Human MS4A1/CD20 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with human MS4A1/CD20 and eGFP was stained with either (A) Mouse Anti-Human MS4A1/CD20 APC-conjugated Monoclonal Antibody (Catalog # FAB4225A) or (B) Mouse IgG1Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.Applications for Human MS4A1/CD20 APC-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: Human peripheral blood lymphocytes and HEK293 human embryonic kidney cell line transfected with human MS4A1/CD20 and eGFP
Sample: Human peripheral blood lymphocytes and HEK293 human embryonic kidney cell line transfected with human MS4A1/CD20 and eGFP
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Background: CD20
CD20 is a 297 amino acid (aa) non-glycosylated type III membrane protein that is the founding member of the MS4A tetraspanning protein family. It is expressed on pre-B, naïve and mature B lymphocytes and B-cell lymphomas. CD20 is associated with lipid rafts upon crosslinking, and appears to play a role in regulation of ion influx. The one extracellular segment of 48 aa shows 65% aa identity between human and mouse CD20.
Long Name
Cluster of Differentiation 20
Alternate Names
B1, Bp35, CD20, LEU-16, Ly-44, MS4A1, S7
Entrez Gene IDs
Gene Symbol
MS4A1
UniProt
Additional CD20 Products
Product Documents for Human MS4A1/CD20 APC-conjugated Antibody
Product Specific Notices for Human MS4A1/CD20 APC-conjugated Antibody
For research use only
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