Human MSK1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2518
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 252608
Product Specifications
Immunogen
E. coli-derived recombinant human MSK1
Met1-Ala802
Accession # O75582
Met1-Ala802
Accession # O75582
Specificity
Detects human MSK1 but not human MSK2 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human MSK1 Antibody
MSK1 in Human Lymphocytes.
MSK1 was detected in immersion fixed human lymphocytes using 8 µg/mL Human MSK1 Monoclonal Antibody (Catalog # MAB2518) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human MSK1 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed human lymphocytes
Sample: Immersion fixed human lymphocytes
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MSK1
Mitogen- and stress-activated protein kinase 1 (MSK1), also known as 90 kDa ribosomal protein S6 kinase 5 (RPS6KA5), belongs to the AGC family of kinases. Both MSK1 and the related MSK2 have two kinase domains connected by a regulatory linker region and are activated by the mitogen-activated protein kinases ERK1, ERK2, and p38. Nuclear MSK phosphorylates and activates a number of transcription factors, including ATF1 and CREB.
Long Name
Mitogen- and Stress-activated Protein Kinase 1
Alternate Names
RPS6KA5
Gene Symbol
RPS6KA5
UniProt
Additional MSK1 Products
Product Documents for Human MSK1 Antibody
Product Specific Notices for Human MSK1 Antibody
For research use only
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