Delects human MSP R/Ron in direct ELISAs and Western blots. In these formats, approximately 25% cross‑reactivity with recombinant mouse MSP R is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human MSPR/Ron PE-conjugated Antibody

Detection of MSP R/Ron in Human Whole Blood Monocytes by Flow Cytometry.

Human whole blood monocytes were stained with Goat Anti-Human MSP R/Ron PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6647P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human MSPR/Ron PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Human whole blood monocytes

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: MSPR/Ron

RON (Recepteur d'Origine Nantais [Receptor originating (or discovered) in Nantes (France)]; also known as MSP-R, PTK8 and MST1R) is a 180-190 kDa member of the tyrosine kinase family, protein kinase superfamily of molecules. Human mature RON is a type I transmembrane heterodimeric glycoprotein that arises from the proteolytic cleavage of a single chain proform 1376 amino acids (aa) in length. Posttranslational processing generates a 35-40 kDa a-chain (aa 25-304) coupled to a 145-150 kDa b-chain (aa 310-1400). The chains are disulfide-linked, and the transmembrane segment appears over aa 958-978. Multiple cell types are known to express RON, and these include osteoclasts, TrkA+ dorsal root ganglia neurons, macrophages, keratinized and nonkeratinized stratified squamous epithelium, intestinal Paneth cells, renal proximal tubule and mesangial cells, monocytes, neutrophils and mammary epithelium. On the cell surface, RON serves as a receptor for MSP, a 78-80 kDa soluble molecule related to HGF. Ligand binding initiates homodimerization and receptor signaling. In addition, RON is also known to heterodimerize with multiple integral membrane proteins, either individually or as part of a complex. Proteins identified to date include b1 integrins, c-MET/HGFR, E-Cadherin, the bc-chain for IL-3, EPO-R, EGFR, IGF-IR, Plexins B1 and B3, and CD44v6. RON may also be found intracellularly, where it interacts with either the androgen receptor or EGFR in the cytoplasm, or in the nucleus, where it acts as a transcription factor coupled to HIF-1a and regulates c-Jun, Bcl-2 and c-FLIP expression. There are about a dozen alternative splice forms for RON, some soluble, while others are transmembrane, and either constitutively active or biochemically inert. They hold the potential to interact with full-length RON and impact its signaling capabilities. Reflecting the diverse nature of RON-expressing cells, RON has been found to generate multiple outcomes, including the promotion of epithelial-to-mesenchymal transitions, the maintenance of M2 macrophage phenotypes, and the initiation of keratinocyte proliferation and migration. Over aa 25-956, human and mouse RON share 76% aa sequence identity.

Long Name

Macrophage Stimulating Protein Receptor

Alternate Names

CD136, MSP R, MST1R, Ron

Entrez Gene IDs

4486 (Human); 19882 (Mouse)

Gene Symbol

MST1R

UniProt

Q04912

Additional MSPR/Ron Products

Product Documents for Human MSPR/Ron PE-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human MSPR/Ron PE-conjugated Antibody

For research use only

Citations
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Flow Cytometry

Human MSPR/Ron PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB6647P

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all MSPR/Ron Antibodies »

Product Specifications

Immunogen

Specificity