Human MST1/STK4 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4939
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human MST1/STK4
Lys285-Asp443
Accession # Q13043
Lys285-Asp443
Accession # Q13043
Specificity
Detects human MST1/STK4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human MST2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human MST1/STK4 Antibody
Detection of Human MST1/STK4 by Western Blot.
Western blot shows lysates of human brain tissue, A172 human glioblastoma cell line, Jurkat human acute T cell leukemia cell line, and SH-SY5Y human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MST1/STK4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4939) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MST1/STK4 at approximately 55-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
MST1/STK4 in SH‑SY5Y Human Cell Line.
MST1/STK4 was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Goat Anti-Human MST1/STK4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4939) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human MST1/STK4 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed SH‑SY5Y human neuroblastoma cell line
Sample: Immersion fixed SH‑SY5Y human neuroblastoma cell line
Western Blot
1 µg/mL
Sample: Human brain tissue, A172 human glioblastoma cell line, Jurkat human acute T cell leukemia cell line, and SH-SY5Y human neuroblastoma cell line
Sample: Human brain tissue, A172 human glioblastoma cell line, Jurkat human acute T cell leukemia cell line, and SH-SY5Y human neuroblastoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 4 reviews rated 3.8 using AF4939 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MST1/STK4
Long Name
Mammalian STE20-like Protein Kinase 1/Serine/Threonine Protein Kinase 4
Alternate Names
KRS2, STK4, YSK3
Gene Symbol
STK4
UniProt
Additional MST1/STK4 Products
Product Documents for Human MST1/STK4 Antibody
Product Specific Notices for Human MST1/STK4 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...