Human MUC-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB62981
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunohistochemistry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2336A
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human MUC-1
Met1-Ser380
Accession # P15941
Met1-Ser380
Accession # P15941
Specificity
Detects human MUC-1 in direct ELISAs.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human MUC-1 Antibody
Detection of MUC-1 in MCF‑7 Human Cell Line by Flow Cytometry.
MCF-7 human breast cancer cell line was stained with Rabbit Anti-Human MUC-1 Monoclonal Antibody (Catalog # MAB62981, filled histogram) or isotype control antibody (MAB1050, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (F0110). Staining was performed using our Staining Membrane-associated Proteins protocol.
MUC‑1 in Human Breast Cancer Tissue.
MUC-1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human MUC-1 Monoclonal Antibody (Catalog # MAB62981) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human MUC-1 Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA detection antibody when paired with rabbit anti-Human MUC-1 monoclonal antibody (Catalog # MAB629812).This product is intended for assay development on various assay platforms requiring antibody pairs.
Flow Cytometry
0.25 µg/106 cells
Sample: MCF-7 human breast cancer cell line
Sample: MCF-7 human breast cancer cell line
Immunohistochemistry
3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MUC-1
Long Name
Mucin 1, Cell Surface-associated
Alternate Names
CD227, Episialin, H23AG, KL-6, Mucin-1, PEM, PEMT
Entrez Gene IDs
4582 (Human)
Gene Symbol
MUC1
UniProt
Additional MUC-1 Products
Product Documents for Human MUC-1 Antibody
Product Specific Notices for Human MUC-1 Antibody
For research use only
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