Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Simple Western, Western Blot

Cited:

Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 604804

View all MUC-1 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human MUC-1
Pro126-Arg145
Accession # P15941

Specificity

Detects human MUC-1 in direct ELISAs and Western blots.  In direct ELISAs, no cross-reactivity with recombinant human (rh) MUC-20, rhMUC-20S, or rhCA125/MUC-16 was observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human MUC-1 Antibody

Detection of Human MUC-1 antibody by Western Blot.

Detection of Human MUC‑1 by Western Blot.

Western blot shows lysates of human lung tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human MUC-1 Monoclonal Antibody (Catalog # MAB6298) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for MUC-1 at approximately 300 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Detection of MUC-1 antibody in MCF-7 Human Cell Line antibody by Flow Cytometry.

Detection of MUC-1 in MCF‑7 Human Cell Line by Flow Cytometry.

MCF-7 human breast cancer cell line was stained with Mouse Anti-Human MUC-1 Monoclonal Antibody (Catalog # MAB6298, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B).
MUC-1 antibody in Capan-1 Human Cell Line by Immunocytochemistry (ICC).

MUC‑1 in Capan‑1 Human Cell Line.

MUC-1 was detected in immersion fixed Capan-1 human pancreatic adenocarcinoma cell line using Mouse Anti-Human MUC-1 Monoclonal Antibody (Catalog # MAB6298) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
View 3 more images

Applications for Human MUC-1 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: MCF‑7 human breast cancer cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed Capan-1 human pancreatic adenocarcinoma cell line

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast

Simple Western

40 µg/mL
Sample: Human lung tissue

Western Blot

2 µg/mL
Sample: Human lung tissue under non-reducing conditions only

Reviewed Applications

Read 1 review rated 5 using MAB6298 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MUC-1

MUC-1 (Mucin-1; also PEM, PEMT, Episialin, tumor-associated mucin, and carcinoma associated mucin) is a 240-450 kDa type I transmembrane glycoprotein. Human MUC-1 is 1255 amino acids (aa) in length and contains a 23 aa signal sequence, a 1135 aa extracellular domain (ECD), a 23 aa transmembrane segment, and a 74 aa cytoplasmic tail. The ECD consists of degenerate tandem repeats and a tandem repeat region, which makes up the major portion of the protein. Potential O-glycosylation sites (serines and threonines) make up more than one-fourth of the amino acids. Splicing variants produce ten isoforms for human MUC-1. Human MUC-1 is 28% aa identical to mouse MUC-1.

Long Name

Mucin 1, Cell Surface-associated

Alternate Names

CD227, Episialin, H23AG, KL-6, Mucin-1, PEM, PEMT

Entrez Gene IDs

4582 (Human)

Gene Symbol

MUC1

UniProt

P15941

Additional MUC-1 Products

Product Documents for Human MUC-1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human MUC-1 Antibody

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.