Human Musashi-2 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF3255

R&D Systems, part of Bio-Techne
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BAF3255
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AF3255
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry, Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all Musashi-2 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Musashi-2 isoform 1
Met1-His328
Accession # Q96DH6

Specificity

Detects human Musashi-2 in Western blots. In Western blots, approximately 15% cross-reactivity with recombinant human Musashi-1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Musashi-2 Biotinylated Antibody

Musashi-2 antibody in MOLT-4 Human Cell Line by Immunocytochemistry (ICC).

Musashi‑2 in MOLT‑4 Human Cell Line.

Musashi-2 was detected in immersion fixed MOLT-4 human acute lymphoblastic leukemia cell line using Goat Anti-Human Musashi-2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3255) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human Musashi-2 Biotinylated Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed MOLT‑4 human acute lymphoblastic leukemia cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Human Musashi-2
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Musashi-2

Musashi-2 belongs to the evolutionarily conserved Musashi family of RNA binding proteins which are involved in the translational control of their target mRNAs. Musashi-2 has two tandem RNA-recognition domains (RRM-1 and RRM-2) that have the highly conserved RNP (ribonucleoprotein) motifs. By alternative splicing, at least three Musashi-2 isoforms exist. In mammalian nervous system, Musashi-1 and –2 are selectively expressed in neural progenitor cells and play important roles in maintenance of the stem cell fate. Human Musashi-2 shares 95% amino acid sequence homology with mouse Musashi-2 and 80% amino acid sequence identity with human Musashi-1. Translocations resulting in the formation of Musashi-2/HOXA9 fusion protein is associated with progression of chronic myelogenous leukemia to the accelerated phase and blast crisis.

Alternate Names

MSI2, Musashi2

Entrez Gene IDs

124540 (Human); 76626 (Mouse)

Gene Symbol

MSI2

UniProt

Q96DH6

Additional Musashi-2 Products

Product Documents for Human Musashi-2 Biotinylated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Musashi-2 Biotinylated Antibody

For research use only

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