Human Myocilin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3446
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Tree Shrew
Applications
Validated:
Immunohistochemistry
Cited:
Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 297817
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Myocilin
Arg33-Met504
Accession # Q99972
Arg33-Met504
Accession # Q99972
Specificity
Detects human Myocilin in direct ELISAs. In direct ELISAs, 75% cross-reactivity with recombinant mouse Myocilin is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Myocilin Antibody
Myocilin in Human Skeletal Muscle.
Myocilin was detected in immersion fixed paraffin-embedded sections of Human Skeletal Muscle using Mouse Anti-Human Myocilin Monoclonal Antibody (Catalog # MAB3446) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sarcoplasm and sarcolemma in skeletal muscle cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human Myocilin Antibody
Application
Recommended Usage
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Human Skeletal Muscle
Sample: Immersion fixed paraffin-embedded sections of Human Skeletal Muscle
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB3446 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Myocilin
Additional Myocilin Products
Product Documents for Human Myocilin Antibody
Product Specific Notices for Human Myocilin Antibody
For research use only
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