Human Nanog PE-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC1997P
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Intracellular Staining by Flow Cytometry
Cited:
Flow Cytometry, Immunocytochemistry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Nanog
Trp153-Val305
Accession # ABZ92376
Trp153-Val305
Accession # ABZ92376
Specificity
Detects human Nanog in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse Nanog is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Nanog PE-conjugated Antibody
Detection of Nanog in BG01V Human Cells by Flow Cytometry.
Three-day Endoderm-differentiated BG01V human embryonic stem cells (upper image) or untreated BG01V human embryonic stem cells (lower image) were stained with Goat Anti-Human Nanog PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC1997P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human Nanog PE-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: BG01V human embryonic stem cells fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: BG01V human embryonic stem cells fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Nanog
References
- Mitsui, K. et al. (2003) Cell 11:631.
- Chambers, I. et al. (2003) Cell 113:643.
- Hart, A.H. et al. (2004) Dev. Dyn. 230:187.
Long Name
Nanog Homeobox
Alternate Names
FLJ12581, FLJ40451, hNanog, homeobox protein NANOG, Homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48, Nanog homeobox
Gene Symbol
NANOG
UniProt
Additional Nanog Products
Product Documents for Human Nanog PE-conjugated Antibody
Product Specific Notices for Human Nanog PE-conjugated Antibody
For research use only
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