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Human Nectin-4 Alexa Fluor® 700-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB2659N

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 337516

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Nectin-4
Gly27-Val351
Accession # Q96NY8

Specificity

Detects human Nectin-4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, does not cross-react with recombinant human Nectin‑1, -2, -3, or recombinant mouse Nectin-4.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Nectin-4 Alexa Fluor® 700-conjugated Antibody

Detection of Nectin-4 antibody in MCF-7 Human Cell Line antibody by Flow Cytometry.

Detection of Nectin-4 in MCF-7 Human Cell Line by Flow Cytometry.

MCF-7 human breast cancer cell line was stained with Mouse Anti-Human Nectin-4 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB2659N, filled histogram) or isotype control antibody (Catalog # IC0041N, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Nectin-4 Alexa Fluor® 700-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: MCF‑7 human breast cancer cell line

Reviewed Applications

Read 1 review rated 5 using FAB2659N in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Nectin-4

Nectin-4 is a 66-70 kDa member of the nectin family, Ig superfamily of molecules. Mature Nectin-4 is a 479 amino acid (aa) Type I transmembrane glycoprotein that contains one V-type and two C2-type Ig-like domains. Nectins mediate the Ca++-independent cell adhesion in junctional complexes and connect to the Actin cytoskeleton via Afadin. Nectins are considered to first form loose cis-homodimers which aggregate and generate homooligomer in-trans, but preferentially generates Nectin-4:Nectin-1 heterooligomers. Cells known to express Nectin-4 include keratinocytes, transitional epithelium, CD49f+ CD24+ luminal mammary epithelium, tooth stratum intermedium cells, and respiratory pseudostratified ciliated columnar epithelium. It is also found on multiple carcinoma cell types. Nectin-4 can be cleaved to generate a 43-44 kDa soluble form that apparently appears only during tumorigenesis. Nectin-4 is also known to bind the measles virus, and possibly interact, indirectly or as a heterodimer, with the prolactin receptor. Over aa 27-351, human and mouse Nectin-4 share 90% aa sequence identity.

Long Name

Poliovirus Receptor Related 4

Alternate Names

LNIR, Nectin4, PRR4, PVRL4

Entrez Gene IDs

81607 (Human); 71740 (Mouse); 498281 (Rat); 102127936 (Cynomolgus Monkey)

Gene Symbol

NECTIN4

UniProt

Additional Nectin-4 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Nectin-4 Alexa Fluor® 700-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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