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Human Nectin-4 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF2659

R&D Systems, part of Bio-Techne
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AF2659
AF2659-SP

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Canine, Hamster, Primate - Chlorocebus aethiops (African Green Monkey), Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Xenograft

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Western Blot

Cited:

Flow Cytometry, Functional Assay, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Paraffin, Neutralization, Westen Blot, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Nectin-4
Gly27-Val351
Accession # Q96NY8

Specificity

Detects human Nectin-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 75% cross‑reactivity with recombinant mouse Nectin-4 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Nectin-4 Antibody

Nectin-4 antibody in Human Placenta by Immunohistochemistry (IHC-P).

Nectin-4 in Human Placenta.

Nectin-4 was detected in immersion fixed paraffin-embedded sections of human placenta using 10 µg/mL Goat Anti-Human Nectin-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2659) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human Nectin-4/PVRL4 by Western Blot

Detection of Human Nectin-4/PVRL4 by Western Blot

Inhibition of Nectin-4 expression by gene silencing decreases cell proliferation in pancreatic cancer cells. (A,B) Capan-2 and BxPC-3 cells were transfected with Nectin-4 siRNA or control RNA. The relative expression of Nectin-4 was significantly reduced in both cells when transfected with Nectin-4 siRNA for up to 72 hours as determined by quantitative real-time PCR and Western blot analysis (n = 4 of each group). (C) Cell proliferation was significantly inhibited by Nectin-4 gene silencing in both cells after 72 hours incubation as determined by MTS assay (n = 6 of each group). *P < 0.001 versus control siRNA (Student’s t test). Image collected and cropped by CiteAb from the following publication (https://jeccr.biomedcentral.com/articles/10.1186/s13046-015-0144-7), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Nectin-4 in MCF-7 Human Cell Line by Flow Cytometry.

MCF-7 human breast adenocarcinoma cell line was stained with Goat Anti-Human Nectin-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2659, filled histogram) or control antibody (AB-108-C, open histogram), followed by PE-conjugated Anti-Goat IgG Secondary Antibody (F0107).

Applications for Human Nectin-4 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: MCF-7 human breast cancer cell line

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human placenta subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Western Blot

0.1 µg/mL
Sample: Recombinant Human Nectin‑4 (Catalog # 2659-N4)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nectin-4

Nectin-4 is a type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. It is both a homophilic and heterophilic (with Nectin-1) cell adhesion molecule that is expressed in the embryo and in breast carcinoma. A soluble form of Nectin-4 is generated from the membrane protein via the action of TACE/ADAM-17. The extracellular domain of human Nectin-4 shares 90% and 92% amino acid sequence homology with the corresponding regions of mouse and rat Nectin-4, respectively.

Long Name

Poliovirus Receptor Related 4

Alternate Names

LNIR, Nectin4, PRR4, PVRL4

Entrez Gene IDs

81607 (Human); 71740 (Mouse); 498281 (Rat); 102127936 (Cynomolgus Monkey)

Gene Symbol

NECTIN4

UniProt

Additional Nectin-4 Products

Product Documents for Human Nectin-4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Nectin-4 Antibody

For research use only

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