Human NEDD9/CASL Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5459

R&D Systems, part of Bio-Techne
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MAB5459
MAB5459-SP
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 584825

View all NEDD9/CASL Antibodies »

Product Summary for Human NEDD9/CASL Antibody

Immunogen

E. coli-derived recombinant human NEDD9/CASL
Lys153-Asn366
Accession # Q14511

Specificity

Detects human NEDD9/CASL in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) NEDD4 or rhNEDD8 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human NEDD9/CASL Antibody

Detection of Human NEDD9/CASL antibody by Western Blot.

Detection of Human NEDD9/CASL by Western Blot.

Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. PVDF Membrane was probed with 0.1 µg/mL of Mouse Anti-Human NEDD9/CASL Monoclonal Antibody (Catalog # MAB5459) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for NEDD9/CASL at approximately 110 kDa & 113 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Human NEDD9/CASL Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NEDD9/CASL

NEDD9 (also HEF1 and CASL) is a 93 kDa (predicted) member of the CAS family of proteins. It is widely expressed, and serves to integrate integrin beta1 and BCR signaling with cell mitotic pathways. Human NEDD9 is 834 amino acids (aa) in length. It contains one SH3 domain (aa 3-65), a Tyr-rich SH2-binding region (aa 90-350), one Ser-rich four alpha-helix bundle protein interaction site (aa 350‑650), and an HLH motif that mediates Cas protein dimerization (aa 710-760). NEDD9 usually runs at 105 or 115 kDa in SDS-PAGE due to phosphorylation. It is also cleaved near Leu361 to generate an active N-terminal 55 kDa fragment. One isoform shows a deletion of aa 155-188, while two others show 20 or 7 aa substitutions for aa 155-834 or 1-153, respectively. Over aa 153-366, human and mouse NEDD9 share 85% aa identity.

Long Name

Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 9

Alternate Names

CAS2, CASL, HEF1, p105

Entrez Gene IDs

4739 (Human); 18003 (Mouse); 291044 (Rat)

Gene Symbol

NEDD9

UniProt

Q14511 (Human)

Additional NEDD9/CASL Products

Product Documents for Human NEDD9/CASL Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human NEDD9/CASL Antibody

For research use only

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