Human NELL1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB54871

R&D Systems, part of Bio-Techne
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MAB54871
MAB54871-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 756435

View all NELL1 Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human NELL1
Arg17-Asn810
Accession # Q92832

Specificity

Detects human NELL1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human NELL2 or recombinant mouse NELL1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human NELL1 Antibody

NELL1 antibody in IMR32 Human Cell Line by Immunocytochemistry (ICC).

NELL1 in IMR32 Human Cell Line.

NELL1 was detected in immersion fixed IMR32 human neuroblastoma cell line using Mouse Anti-Human NELL1 Monoclonal Antibody (Catalog # MAB54871) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human NELL1 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed IMR32 human neuroblastoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NELL1

NELL1 (neural EGF-like like protein 1) is an 89 kDa (predicted) member of the EGF-like domain containing family, Laminin G/N-TSP1/Pentraxin gene superfamily of molecules. When secreted, NELL1 exists as a phosphoglycoprotein that can add as much as 50 kDa to the calculated MW. NELL1 has restricted expression, being limited to pre-B cells and osteoblasts, where it apparently promotes osteoblast maturation and bone formation. In tumors, it is found in neuroblastoma-derived cells. NELL1 is both secreted and retained intracellularly where it is phosphorylated by PKC. The human NELL1 precursor is 810 amino acids (aa) in length. It contains a 16 aa signal sequence plus a 794 aa mature region. The mature region possesses an N-terminal TSP domain (aa 81-230), two VWFC domains (aa 271-390), six consecutive EGF-like domains (aa 391-631), and three additional C-terminal VWFC domains (aa 632-807). Secreted NELL1 forms a 400-420 kDa noncovalent homotrimer. Over aa 17-810, human NELL1 shares 93% aa identity with mouse and rat NELL1. Alternate splicing generates an additional isoform of human NELL1 that lacks the fifth EGF-like domain.

Long Name

NEL-like 1

Alternate Names

IDH3GL

Entrez Gene IDs

4745 (Human); 338352 (Mouse)

Gene Symbol

NELL1

UniProt

Q92832

Additional NELL1 Products

Product Documents for Human NELL1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human NELL1 Antibody

For research use only

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