Human Neprilysin/CD10 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1182
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 212504
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Neprilysin/CD10
Met45-Pro743
Accession # P08473
Met45-Pro743
Accession # P08473
Specificity
Detects human Neprilysin/CD10 in Western blots and direct ELISAs. In Western blots, less than 5% cross-reactivity with recombinant human ECE-2 and recombinant mouse Neprilysin is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Neprilysin/CD10 Antibody
Detection of Neprilysin/CD10 in Daudi Human Cell Line by Flow Cytometry.
Daudi human Burkitt's lymphoma cell line was stained with Mouse Anti-Human Neprilysin/CD10 Monoclonal Antibody (Catalog # MAB1182, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).Applications for Human Neprilysin/CD10 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Daudi human Burkitt's lymphoma cell line
Sample: Daudi human Burkitt's lymphoma cell line
Western Blot
1 µg/mL
Sample: Recombinant Human Neprilysin/CD10 (Catalog # 1182-ZNC)
Sample: Recombinant Human Neprilysin/CD10 (Catalog # 1182-ZNC)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Neprilysin/CD10
Neprilysin (NEP) is also known as CALLA and CD10. It is a zinc metalloprotease expressed at the cell surface that cleaves a variety of bioactive peptides such as enkephalins, atrial natriuretic peptide and amyloid beta peptide.
Alternate Names
CALLA, CD10, Enkephalinase, Leu-19, MME, Neutral Endopeptidase 24.11, NKH1
Gene Symbol
MME
UniProt
Additional Neprilysin/CD10 Products
Product Documents for Human Neprilysin/CD10 Antibody
Product Specific Notices for Human Neprilysin/CD10 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...