Human Neprilysin/CD10 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF1182

R&D Systems, part of Bio-Techne
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AF1182
AF1182-SP
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Conjugate
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Biotin
BAF1182
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

CyTOF-ready, ELISA Capture (Matched Antibody Pair), Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot

Cited:

Activity Assay, ELISA Development, Immunocytochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all Neprilysin/CD10 Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Neprilysin/CD10
Tyr52-Trp750
Accession # P08473

Specificity

Detects human Neprilysin/CD10 in ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Neprilysin/CD10 Antibody

Neprilysin/CD10 antibody in Human Kidney by Immunohistochemistry (IHC-P).

Neprilysin/CD10 in Human Kidney.

Neprilysin/CD10 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human Neprilysin/CD10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1182) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human and Mouse Neprilysin/CD10 antibody by Western Blot.

Detection of Human and Mouse Neprilysin/CD10 by Western Blot.

Western blot shows lysates of LNCaP human prostate cancer cell line and PC-3 human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Neprilysin/CD10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1182) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). For additional reference, Recombinant Mouse Neprilysin (1126-ZN) (5 and 50 ng/lane) and Recombinant Human Neprilysin (CHO-expressed) (1182-ZNC) (5 and 50 ng/lane) were included. Specific bands were detected for Neprilysin/CD10 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse Neprilysin/CD10 by Western Blot

Detection of Mouse Neprilysin/CD10 by Western Blot

Effects of A beta1-42 and Nav beta2 on APP, BDNF, and NEP in A beta1-42-treated mice. (a) Representative blots of Nav beta2, APP, BDNF, NEP, and GAPDH in mice with various treatments indicated by Western blot assay. (b–e) Quantitative plots of Nav beta2, APP, BDNF, and NEP in hippocampus of mice from different groups. (f) Quantitative image of NEP enzymatic activity between each experimental group. Intragroup sample size n = 7. ∗Versus two different treatment groups, P < 0.05. #Compare the remaining treatment groups with the marker group, P < 0.05. −Versus two different treatment groups, P > 0.05. Empty: empty viral vector; OE: overexpression; si: small interfering. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36406589), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Neprilysin/CD10 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: CD19+ human peripheral blood lymphocytes

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Neprilysin/CD10 (Catalog # 1182-ZNC), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: LNCaP human prostate cancer cell line, PC‑3 human prostate cancer cell line, Recombinant Human Neprilysin (CHO-expressed) (Catalog # 1182-ZNC), and Recombinant Mouse Neprilysin (Catalog # 1126-ZN)

Human Neprilysin/CD10 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 0.2-0.8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human Neprilysin/CD10 Biotinylated Antibody (Catalog # BAF1182)
  • Standard: Recombinant Human Neprilysin (CHO-expressed) Protein, CF (Catalog # 1182-ZNC)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neprilysin/CD10

Neprilysin/CD10, also known as NEP and neutral endopeptidase 24.11, is a zinc metallopeptidase expressed at the cell surface of a variety of cells. The enzyme functions both as an endopeptidase with a thermolysin-like specificity and as a dipeptidylcarboxypeptidase. NEP has been shown to be involved in the degradation of enkephalins in the mammalian brain and the inactivation of circulating atrial natriuretic peptide (1, 2). NEP has also been identified as the common acute lymphocytic leukemia antigen (CALLA), and is expressed on the surface of lymphocytes in some disease states (3, 4). These and other observations have resulted in considerable interest in NEP as a target for analgesics and antihypertensive drugs. NEP is also a major degrading enzyme of amyloid beta peptide (A beta) in the brain, indicating that down-regulation of NEP activity, which could be caused by aging, can contribute to the development of Alzheimer’s disease by promoting A beta accumulation (5).

References

  1. Malfroy, B. et al. (1978) Nature 276:523.
  2. Kenny, A.J. and Stephenson, S.L. (1988) FEBS Lett. 232:1.
  3. LeTarte, M. et al. (1988) J. Exp. Med. 168:1247.
  4. Shipp, M.A. et al. (1988) Proc. Natl. Acad. Sci. USA 85:4819.
  5. Itwata, N. et al. (2001) Science 292:1550.

Alternate Names

CALLA, CD10, Enkephalinase, Leu-19, MME, Neutral Endopeptidase 24.11, NKH1

Entrez Gene IDs

4311 (Human); 17380 (Mouse); 24590 (Rat); 101925772 (Cynomolgus Monkey)

Gene Symbol

MME

UniProt

P08473

Additional Neprilysin/CD10 Products

Product Documents for Human Neprilysin/CD10 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Neprilysin/CD10 Antibody

For research use only

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