Human Neuregulin-1/NRG1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB377
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Rat
Applications
Validated:
Western Blot
Cited:
ELISA Development (Capture), Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 147724
Product Specifications
Immunogen
E. coli-derived recombinant human Neuregulin‑1/NRG1
Ser2-Lys246
Accession # AAA58639
Ser2-Lys246
Accession # AAA58639
Specificity
Detects human Neuregulin‑1/NRG1 in direct ELISAs and Western blots. In direct ELISAs, no cross‑reactivity with the EGF‑like domains of recombinant human (rh) NRG1-beta 1 (aa 176‑246) or rhNRG1-alpha (aa 177‑241). Therefore, this antibody recognizes an epitope located within aa 2‑176 segment N‑terminal to the EGF‑like domain. This segment is present in all NRG1‑ alpha, beta and gamma isoforms and in NDF43 and glial growth factor (GGF). It is absent from the NRG1 gene products GGF2 and SMDF.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications for Human Neuregulin-1/NRG1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human Neuregulin-1/NRG1 (Catalog # 5898-NR)
Sample: Recombinant Human Neuregulin-1/NRG1 (Catalog # 5898-NR)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Neuregulin-1/NRG1
References
- Buonanno, A., and G.D Fischbach (2001) Curr. Opin. Neurobiol. 11:287.
- Adlkofer, K. and C. Lai (2000) Glia 29:104.
- Garratt, A.N. et al., (2000) BioEssays 22:987.
Alternate Names
Heregulin-1, HGL, HRG1-alpha, HRG1-beta 1, Neuregulin1, NRG1
Gene Symbol
NRG1
UniProt
Additional Neuregulin-1/NRG1 Products
Product Documents for Human Neuregulin-1/NRG1 Antibody
Product Specific Notices for Human Neuregulin-1/NRG1 Antibody
For research use only
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