Human Neuroglycan C/CSPG5 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5685

R&D Systems, part of Bio-Techne
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AF5685
AF5685-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all Neuroglycan C/CSPG5 Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Neuroglycan C/CSPG5
Val31-Gln420
Accession # AAQ04776

Specificity

Detects human Neuroglycan C/CSPG5 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross‑reactivity with recombinant mouse Neuroglycan C is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Neuroglycan C/CSPG5 Antibody

Detection of Human Neuroglycan C/CSPG5 antibody by Western Blot.

Detection of Human Neuroglycan C/CSPG5 by Western Blot.

Western blot shows lysates of SH-SY5Y human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Neuroglycan C/CSPG5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5685) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Neuroglycan C/CSPG5 at approximately 120 and 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human Neuroglycan C/CSPG5 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse brain (cerebellum)

Western Blot

1 µg/mL
Sample: SH-SY5Y human neuroblastoma cell line

Reviewed Applications

Read 1 review rated 5 using AF5685 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neuroglycan C/CSPG5

Neuroglycan C (NGC; also CSPG5 and CALEB) is a 120‑150 kDa type I transmembrane glycoprotein and member of the neuregulin family of proteins (1, 2). Depending on its expression profile, NGC may be a glycoprotein of 120 kDa, or a chondroitin sulfate (CS) proteoglycan of 150 kDa (2, 3). Human NGC is synthesized as a 566 amino acid (aa) precursor that contains a 30 aa signal sequence, a 393 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 122 aa cytoplasmic region. The ECD contains one CS attachment domain (aa 34‑272), with CS attachment at Ser117, one EGF‑like domain (aa 371‑413), two potential sites for N‑linked glycosylation, and twelve potential sites for O‑linked glycosylation (4). Splicing variants produce three isoforms for human NGC. Isoform 1 is the long form. Isoform 2 has a deletion of aa 487‑513, while isoform 3 has an alternative start site at Met139 and the same deletion. Phosphorylation likely occurs at Ser249, and proteolysis generates a 75 kDa soluble fragment (5). Over aa 31‑420, human NGC shares 84% aa identity with mouse NGC. NGC is expressed in nervous tissue and is found on retinal ganglion cells, cerebellar Purkinje cells and hippocampal neurons (6). NGC may function as a growth and differentiation factor involved in neuritogenesis. One study shows that the recombinant ectodomain of NGC core protein enhances neurite outgrowth from rat neocortical neurons in culture via phosphatidylinositol 3‑kinase and protein kinase C signaling pathways (7). Another study states that NGC is a novel component of midkine receptors, a heparin‑binding growth factor that promotes cell attachment and process extension in oligodendroglial precursor‑like cells (3). NGC also acts as a growth factor by directly binding ERbB3 tyrosine kinase and transactivating ErbB2 (1).

References

  1. Kinugasa, Y. et al. (2004) Biochem. Biophys. Res. Commun 321:1045.
  2. Yasuda, Y. et al. (1998) Neurosci. Res. 32:313.
  3. Ichihara-Tanaka, K. et al. (2006) J. Biol. Chem. 281:30857.
  4. Aono, S. et al. (2004) J. Biol. Chem. 279:46536.
  5. Shuo, T. et al. (2007) J. Neurochem. 102:1561.
  6. Aono, S. et al. (2006) J. Neurosci. Res.83:110.
  7. Nakanishi, K. et al. (2006) J. Biol. Chem. 281:24970.
 

Alternate Names

CALEB, CSPG5, NGC

Entrez Gene IDs

10675 (Human); 29873 (Mouse); 50568 (Rat)

Gene Symbol

CSPG5

UniProt

AAQ04776

Additional Neuroglycan C/CSPG5 Products

Product Documents for Human Neuroglycan C/CSPG5 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human Neuroglycan C/CSPG5 Antibody

For research use only

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