Human Neuromedin BR/NMBR Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB47281
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 466501
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human Neuromedin B R/NMBR
Accession # NP_002502
Accession # NP_002502
Specificity
Detects human Neuromedin B R/NMBR in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Neuromedin BR/NMBR Antibody
Neuromedin B R/NMBR in Human Colon Cancer Tissue.
Neuromedin B R/NMBR was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Human Neuromedin B R/NMBR Monoclonal Antibody (Catalog # MAB47281) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Neuromedin BR/NMBR Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human colon cancer tissue
Reviewed Applications
Read 1 review rated 5 using MAB47281 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Neuromedin BR/NMBR
Neuromedin B Receptor (NMBR) is an 80 kDa, 390 amino acid (aa) G-protein coupled 7-transmembrane glycoprotein receptor for bombesin-like peptides, binding Neuromedin B with high affinity and GRP (gastrin releasing peptide) with lower affinity. Neuromedin B R expression in the olfactory and central thalamic regions of the brain plays a role in thermoregulation. It has also been shown to be mitogenic in colonic epithelium. Expression in the pituitary gland is important for regulation of the pituitary-thyroid axis. Within extracellular domains, human Neuromedin B R shares 86% and 82% aa identity with mouse and rat Neuromedin B R, respectively.
Long Name
Neuromedin B Receptor
Alternate Names
Neuromedin B R, NMBR
Gene Symbol
NMBR
UniProt
Additional Neuromedin BR/NMBR Products
Product Documents for Human Neuromedin BR/NMBR Antibody
Product Specific Notices for Human Neuromedin BR/NMBR Antibody
For research use only
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