Human Neurotensin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11548
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 1075611
Product Specifications
Immunogen
Neurotensin containing synthetic peptide
Accession # P30990
Accession # P30990
Specificity
Detects a synthetic peptide around aa 135 in Direct ELISA
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Neurotensin Antibody
Detection of Neurotensin in Human Small Bowel.
Neurotensin was detected in immersion fixed paraffin-embedded sections of human small bowel using Mouse Anti-Human Neurotensin Monoclonal Antibody (Catalog # MAB11548) at 3 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of neuroendocrine cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Neurotensin Antibody
Application
Recommended Usage
Immunohistochemistry
3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human small bowel
Sample: Immersion fixed paraffin-embedded sections of human small bowel
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute lyophilized material at 0.2mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Neurotensin
References
- Torruella-Suárez ML, McElligott ZA. Neurotensin in reward processes. Neuropharmacology. 2020 May 1;167:108005. doi: 10.1016/j.neuropharm.2020.108005. Epub 2020 Feb 11. PMID: 32057800; PMCID: PMC7238864.
- Mazella J, Béraud-Dufour S, Devader C, Massa F, Coppola T. Neurotensin and its receptors in the control of glucose homeostasis. Front Endocrinol (Lausanne). 2012 Nov 26;3:143. doi: 10.3389/fendo.2012.00143. PMID: 23230428; PMCID: PMC3515879.
Long Name
Neurotensin/neuromedin N [Cleaved into: Large neuromedin N
Alternate Names
NTS
Gene Symbol
NTS
UniProt
Additional Neurotensin Products
Product Documents for Human Neurotensin Antibody
Product Specific Notices for Human Neurotensin Antibody
For research use only
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