Human Niemann-Pick Type C2/NPC2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF8644
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf21-derived recombinant human Niemann-Pick Type C2/NPC2
Glu20-Leu151
Accession # P61916
Glu20-Leu151
Accession # P61916
Specificity
Detects human Niemann-Pick Type C2/NPC2 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human Niemann-Pick Type C2/NPC2 Antibody
Detection of Human Niemann-Pick Type C2/NPC2 by Western Blot.
Western blot shows lysates of NTera-2 human testicular embryonic carcinoma cell line, human kidney (cortex) tissue, HepG2 human hepatocellular carcinoma cell line, and human skeletal muscle tissue. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human Niemann-Pick Type C2/NPC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8644) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Niemann-Pick Type C2/NPC2 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Niemann-Pick Type C2/NPC2 in SK-Hep-1 Human Cell Line.
Niemann-Pick Type C2/NPC2 was detected in immersion fixed SK-Hep-1 human liver adenocarcinoma cell line using Rabbit Anti-Human Niemann-Pick Type C2/NPC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8644) at 1:150 dilution for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Niemann-Pick Type C2/NPC2 in Human Kidney.
Niemann-Pick Type C2/NPC2 was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human Niemann-Pick Type C2/NPC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8644) at 1:50 dilution overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in tubular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Niemann-Pick Type C2/NPC2 Antibody
Application
Recommended Usage
Immunocytochemistry
1:150 dilution
Sample: Immersion fixed SK-Hep-1 human liver adenocarcinoma cell line
Sample: Immersion fixed SK-Hep-1 human liver adenocarcinoma cell line
Immunohistochemistry
1:50 dilution
Sample: Immersion fixed paraffin-embedded sections of human kidney
Sample: Immersion fixed paraffin-embedded sections of human kidney
Western Blot
1:1000 dilution
Sample: NTera‑2 human testicular embryonic carcinoma cell line, human kidney (cortex) tissue, and HepG2 human hepatocellular carcinoma cell line
Sample: NTera‑2 human testicular embryonic carcinoma cell line, human kidney (cortex) tissue, and HepG2 human hepatocellular carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using AF8644 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
For liquid material, refer to CoA for concentration.
Formulation
Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
- 12 months from date of receipt, -20 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after opening.
- 6 months, -20 °C under sterile conditions after opening.
Background: Niemann-Pick Type C2/NPC2
Alternate Names
EDDM1, Epididymal Protein 1, HE1, NiemannPick Type C2, NPC2
Gene Symbol
NPC2
UniProt
Additional Niemann-Pick Type C2/NPC2 Products
Product Documents for Human Niemann-Pick Type C2/NPC2 Antibody
Product Specific Notices for Human Niemann-Pick Type C2/NPC2 Antibody
* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...