Human Ninjurin-1 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF5105
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Biotin
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Ninjurin-1
Asp2-Val81
Accession # Q92982
Asp2-Val81
Accession # Q92982
Specificity
Detects human Ninjurin‑1 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human Ninjurin-2 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Ninjurin-1 Biotinylated Antibody
Detection of Human Ninjurin‑1 by Western Blot.
Western blot shows lysates of A549 human lung carcinoma cell line and LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Ninjurin-1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF5105) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Ninjurin-1 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Ninjurin-1 Biotinylated Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: A549 human lung carcinoma cell line and LNCaP human prostate cancer cell line
Sample: A549 human lung carcinoma cell line and LNCaP human prostate cancer cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Ninjurin-1
Ninjurin-1 (nerve injury-induced protein 1) is a 20-22 kDa member of the Ninjurin family of transmembrane (TM) proteins. It is expressed by Schwann cells, neurons and hepatocytes and participates in intercellular homophilic binding during nerve regeneration. Human Ninjurin-1 is 152 amino acids in length. It has an unusual membrane orientation. There is an 80 amino acid (aa) N-terminal extracellular domain (ECD) (aa 1-80), followed by a TM segment, a cytoplasmic region, a second TM segment and a C-terminal ECD (aa 142-152). Homophilic binding is divalent-cation dependent and occurs between Pro26 and Asn37. Over aa 1-80, human Ninjurin-1 shares 84% aa sequence identity with mouse Ninjurin-1. Human Ninjurin-1 shares only 50% aa sequence identity with human Ninjurin-2.
Alternate Names
NIN1, NINJ1, Ninjurin1
Gene Symbol
NINJ1
UniProt
Additional Ninjurin-1 Products
Product Documents for Human Ninjurin-1 Biotinylated Antibody
Product Specific Notices for Human Ninjurin-1 Biotinylated Antibody
For research use only
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