Human NKX2.5 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2444
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Rat
Applications
Validated:
Immunohistochemistry
Cited:
Immunocytochemistry, Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 259416
Product Specifications
Immunogen
E. coli-derived recombinant human NKX2.5
Gln24-Arg137
Accession # P52952
Gln24-Arg137
Accession # P52952
Specificity
Detects human NKX-2.5 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human NKX2.5 Antibody
NKX2.5 in Human Heart.
NKX2.5 was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human NKX2.5 Monoclonal Antibody (Catalog # MAB2444) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human NKX2.5 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human heart and perfusion fixed frozen sections of rat heart
Sample: Immersion fixed paraffin-embedded sections of human heart and perfusion fixed frozen sections of rat heart
Reviewed Applications
Read 1 review rated 4 using MAB2444 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NKX2.5
NKX2.5, also called NKX-2E or Csx (Cardiac specific homeobox protein), is a transcriptional activator. Its expression is essential for normal cardiovascular development. During embryonic development, NKX2.5 is also expressed in the foregut, thyroid, spleen, and stomach, while in the adult expression is predominantly restricted to the heart.
Long Name
NK2 Homeobox 5
Alternate Names
CSX1, NKX2-5, NKX2E
Entrez Gene IDs
1482 (Human)
Gene Symbol
NKX2-5
UniProt
Additional NKX2.5 Products
Product Documents for Human NKX2.5 Antibody
Product Specific Notices for Human NKX2.5 Antibody
For research use only
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